Total Flavonoids Content was Improved by Chalcone Synthase Overexpression in Callus Cell of Fagopyrum tataricum

Abstract:

According to the online software, the primer is designed by CODEHOPE(Consensus De-generate Hybrid Oligonucleotide Primers),then the cDNA sequence of Chalcone synthase was obtained by the method of RACE(GenBank No. HQ434624).Then the high efficiency vector named pCM-BCHS was constructed by pCAMBIA1301 and Chalcone synthase gene. The vector was transformed by Agrobacterium tumefaciens to buckwheat induced callus cell. At last the selected transgene callus cell can synthase the total flavonoid at 48.3mg/g DW, which is two times of the wild type. Therefore, it is proved that the overexpression of Chalcone synthase will enhance the metablic flux of total flavonoid synthesis.

• Periodical: Advanced Materials Research (Volumes 781-784)
• Pages: 1755-1759
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.781-784.1755
• Online since: September 2013
• Authors: Kai Liu, Guan Wang, Han Song Yu, Yao Hui Hu
• Keywords: Buckwheat Callus, Chalcone Synthase, Content of Total Flavonoid

References

[1] Xiaochen Gu. [J]. Food and Feeds industry, 1999, (7): 19-23. (in Chinese).

[2] Rufa Lin, Chinese Buckwheat. Beijing: China Agricultural publisher, 1994. (in Chinese).

[3] MENG M. L., WANG H. Z. J. Pract Diabet, 2000, 8(3): 22-23(in Chinese).

[4] LIN H. S.J. Chin. Integrative Med, 2004, 2(1): 72-73(in Chinese).

[5] HE J, KLAG M J, WHELTON M J, et al. Am.J. Clin. Nutr, 1995, 61: 366.

[6] Koes RE, Quattrocchio F, Mol JN. BioEssays, 1994, 16: 123-132.

[7] Winkel- Shirley B. CurrentOpinion Plant Biology, 2002, 5(3): 218-223.

[8] Hansong Yu, Jixing Zhang, Lanqing Ma. Journal of JLAU, 2008, 30(2): 150-153. (in Chinese).

[9] ROSE T, SCHULTZ E, HENIKOFF J, et al. Nucleic Acids Res, 1998, 26(7): 1628-1635.

[10] Hansong Yu, Jixing Zhang, Lanqing Ma, Yaohui Hu. Food Science, 2010, 31(21): 244-247. (in Chinese).

[11] SAMBROOK J, RUSSELL D W. Molecular cloning: a laboratory manual[M]. 3rd edition. New York: Cold Spring Harbor Laboratory Press, (2001).

[12] Lanz T, TroPf S, Marner F-J, Sehröder J, Sehröder G. J Biol Chem，1991, 266: 9971-9976.

[13] Jez J M and Noel J P. J Biol Chem, 2000, 275: 39640-3964.

[14] Schubert S Y, Lansky E P，Neeman I. Jounal of EthnoPharmacology, 1999, 66(l): 11-17.

[15] Ohara T, Ohinata H, Muramatsu N. Nippon Shokuhin Kogyo Gakkaishi, 1989, 36(2): 121-126.