Performance of Indirect ELISA for Maize Hybrid Purity Test

Yue Hui Liu; Jun Wang; Xin Chao Yang; Na Xin Sun; Li Hua Xu

doi:10.4028/www.scientific.net/AMR.781-784.1741

Paper Titles

Preparation of Antibodies against Maize Inbred Lines Storage Proteins
p.1717

Effect of Different Factors on the Viscosity of Collagen from Amiurus nebulosus Skin
p.1723

Isolation and Characterization of Acid-Soluble Collagen from the Skin of Amiurus nebulosus
p.1728

Effect of Coconut Water on the Growth of Gluconacetobacter xylinus Y15
p.1736

Performance of Indirect ELISA for Maize Hybrid Purity Test
p.1741

Accumulation of Copper, Cadmium, Lead and Zinc in Various Tissues of the River Crab from Northeast China
p.1745

Optimization of Extraction Process for Carotenoids from Tamarillo Fruits by Response Surface Methodology
p.1749

Total Flavonoids Content was Improved by Chalcone Synthase Overexpression in Callus Cell of Fagopyrum tataricum
p.1755

Characteristics of Soybean Meals by Solid State Fermentation Using Bacillus subtilis GA15
p.1760

HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 781-784Performance of Indirect ELISA for Maize Hybrid...

Performance of Indirect ELISA for Maize Hybrid Purity Test

Abstract:

Maize is the second large crop in China that needed 100-120 million tons seeds for an average year and the production loss caused by inferior seeds was amazing. Bing an allogamous wind-pollinated plant, maize hybrid purity problem is mainly reflected the inbred lines seed mixed in hybrid. Therefore it is important to have specific and sensitive purity test methods to prevent the poor seeds into market. In this paper, we developed an inderect ELISA using polyconal antibody against maize inbred lines to bind extracts from whole grain with pure water. The single blind trial demonstrated that the average detectable rate of indrect ELISA was 95.2%. We also extended this method to other maize hybrid varieties, found it has no ability to descriminate the maize hybrid facticity.

You might also be interested in these eBooks

View Preview

Info:

Periodical:

Advanced Materials Research (Volumes 781-784)

Pages:

1741-1744

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.781-784.1741

Citation:

Cite this paper

Online since:

September 2013

Authors:

Yue Hui Liu, Jun Wang, Xin Chao Yang, Na Xin Sun, Li Hua Xu

Keywords:

Indirect ELISA, Maize Hybrid, Purity Test

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

Citation:

References

[1] Dominique Dietiker, Peter Stamp, Werner Eugster, Predicting seed admixture in maize combining flowering characteristics and a Lagrangian stochastic dispersion model, Field Crops Research. 121(2011), 256-267.

DOI: 10.1016/j.fcr.2010.12.009

Google Scholar

[2] C. Q. Bo, S. Q. Jin, Z. J. Liang, The seed test technology report, China Agricultural Technology Extension. 26(2006), 276-279 (in Chinese).

Google Scholar

[3] State Bureau of Technical Supervision. Crops seed test procedure. Beijing, (2005).

Google Scholar

[4] Y. Y. Huang, L. W. Zhu, J. H. Li, J. H. Wang, B. Q. Sun, Q. Sun, Rapid and nondestructive discrimination of hybrid maize seed purity using near infrareds pectroscopy, Spectroscopy and Spectral Analysis. 31(2011), 661~664 (in Chinese).

Google Scholar

[5] H. N. Gao, E. H. Zhang, D. R. Li, Research on SSR molecular marker indentification technique of hybrids purity in cabbage, Journal of Northwest A&F Unifersity (Nat. Sci. Ed. ). 5(2012), 117~122 (in Chinese).

Google Scholar

[6] L. M. Luo, L. Liu, L. J. Yu, Application of molecular marker technology in seed purity identification of maize, Current Biotechnology. 1(2011), 7-13 (in Chinese).

Google Scholar

[7] Brink D. E., Prince S. C., Monoclonal antibodies against zeins, Seed Sci. & Technol. . 17(1989), 1-6.

Google Scholar

[8] C. Q. Zhang, X. K. Jin, C.C. Zheng, NAU-PAGE technology and purity test of maize seed, Scientia Agricultura Sinica. 28(1995), 202-206 (in Chinese).

Google Scholar

[9] Jamesma. Conroy, A. Asim Esen, An enzyme-linked immunosorbent assay for zeins and other prolamins. Using unconventional solvents for antigen adsorption, Anal. Biochem. . 137(1983), 182-187.

DOI: 10.1016/0003-2697(84)90368-3

Google Scholar

[10] Robert Hnasko, Alice lin, Jeffery A. McGarvey, Larry H. Stanker, A rapid method to improve protein detection by indirect ELISA, Biochem. Biophys. Res. Commun. . 410 (2011), 726-731.

DOI: 10.1016/j.bbrc.2011.06.005

Google Scholar