Study of Ultrasonic-Freeze-Thaw-Cycle Assisted Extraction of Polysaccharide and Phycobiliprotein from Gracilaria lemaneiformis

Abstract:

Ultrasonic-freeze-thaw-cycle assisted extraction was used to synchronously extract polysaccharide and phycobiliprotein from Gracilaria lemaneiformis. Firstly, different solvents (deionized water, sodium hydroxide, phosphate buffer solution (PBS) and calcium chloride solution) were screened, and PBS (1 mM, pH 6.8) was considered as the best solvent for its OD₅₆₅ value (0.086±0.0003) and OD₅₉₅ value (0.471±0.002), which was significantly higher than any other solvents (p<0.05). Moreover, an L₉ (3)⁴ orthogonal test was carried out to optimize extraction conditions, based on the single-factor test. The results indicated that the optimized conditions were fixed as follows: the ratio of solid to liquid 1:25 (w/v), 2 times of freeze-thaw cycles, ultrasound power 320 W and ultrasound time 10 min, which obtained the optimum yield and purity of polysaccharide and phycobiliprotein, 30.54%, 1.36% and 0.296, respectively. As a result, ultrasonic-freeze-thaw-cycle assisted extraction is considered to be an effective, economic and eco-environmental method to extract polysaccharide and phycobiliprotein.

• Periodical: Advanced Materials Research (Volumes 781-784)
• Pages: 1818-1824
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.781-784.1818
• Online since: September 2013
• Authors: Ming Qiu Liu, Xian Qing Yang, Bo Qi, Lai Hao Li, Jian Chao Deng, Xiao Hu
• Keywords: Gracilaria lemaneiformis, Phycobiliprotein, Polysaccharide, Ultrasonic-Freeze-Thaw-Cycle Assisted Extraction

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