Identification and Primary Application of Two High-Yield Glucoamylase Moulds from Luzhou-Flavor Daqu

Guang Bin Ye; Ming Hong Hong; Dan Huang; Xiao Dong Yang

doi:10.4028/www.scientific.net/AMR.781-784.1825

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 781-784Identification and Primary Application of Two...

Identification and Primary Application of Two High-Yield Glucoamylase Moulds from Luzhou-Flavor Daqu

Abstract:

Six moulds were isolated from Luzhou-flavor Daqu through traditional microorganism isolation method. Among them, two strains njsys-3 and njsys-6 have shown higher glucoamylase activity, their enzyme activity were 1842 U/g and 2349U/g separately. Molecular biological identification results shown that strain njsys-3 was Aspergillus clavatus and strain njsys-6 was Aspergillus niger. Daqu-making test showed that once the inoculating amount of seeding broth of njsys-3 attached to 6 ‰, the glucoamylase activity of Daqu can increase 26.9%; when the inoculating amount of seeding broth of njsys-6 attached to 6 ‰, the activity of glucoamylase of Daqu can increase 34.1% and the fragrance of Daqu can increase.

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Periodical:

Advanced Materials Research (Volumes 781-784)

Pages:

1825-1829

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.781-784.1825

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Online since:

September 2013

Authors:

Guang Bin Ye, Ming Hong Hong, Dan Huang, Xiao Dong Yang

Keywords:

Glucoamylase, Luzhou-Flavor DaQu, Molecular Biological Identification, Mould

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