The New Wheat MDH-GOAT Enzyme Complex and its Application for Quantitative Determination of Glutamate Concentration

Abstract:

The quantitative determination of glutamate is very important for diagnostic of many diseases of the nervous system, severity of stroke of the brain and also for determination glutamate in the food stuffs [1,2]. To the present days for quantitative determination of glutamate is wide used to the enzyme preparation of glutamate dehydrogenase (GDh) from bovine liver. However the application of GDh has the next serious disadvantages: high lability of this preparation and many substances strong change the activity of GDh. For example there are nucleotides, amino acid, steroid and metal ions. As the results the application of GDh preparation dont give reliable determination of quantity of glutamate. Thus there is the high necessity to propose another enzyme preparation for quantitative determination of glutamate without above mentioned disadvantages. In this reason we propose absolutely new enzyme preparation for this aim. In the laboratory of the enzyme structure and regulation of the Institute of the molecular biology and biochemistry of the Ministry of the education and science of the Republic of Kazakhstan was discovered the new enzyme complex (EC), which consists of malate dehydrogenase (MDh) and glutamate-oxaloacetate aminotransferase (GOAT) and used it for quantitative determination of glutamate concentration [3].

• Periodical: Advanced Materials Research (Volumes 781-784)
• Pages: 957-960
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.781-784.957
• Online since: September 2013
• Authors: Ibragimova Saniyam, Elena Gukkengeimer, Nataliya Riger, Gulzat Kulbaeva, Murat Gilmanov
• Keywords: Diseases of the Nervous System, Enzyme Complex, Foodstuffs, Glutamate, Malate Dehydrogenase

References

[1] Heather Scott Walton and Peter R. Dodd Glutamate /glutamine cycling in Alzheimer's disease /Neurochemistry International Volume 50, Issues 7-8, June 2007, Pages 1052-1066 Mechanisms of Neurodegeneration.

DOI: 10.1016/j.neuint.2006.10.007

[2] Cornelia Poulopoulou, Ioannis Markakis, Panagiota Davaki, Eleftheria Tsaltas, Antonis Rombos, Alexandros Hatzimanolis and Dimitrios Vassilopoulos / Aberrant modulation of a delayed rectifier potassium channel by glutamate in Alzheimer's disease/ Neurobiology of Disease Volume 37, Issue 2, February 2010, Pages 339-348 Frontiers in Brain Repair.

DOI: 10.1016/j.nbd.2009.10.012

[3] M.K. Gilmanov, Zh.S. Kudiyarova, Zh.K. Rakhmetova, A.S. Koldasova Study of physical - chemical properties of a new enzyme complex MDH-GOAT in cereal plants / KazNU Biology Series. - 2006. - № 4 (30). - S. 37-43.

[4] Karina E. J. Trípodi and Florencio E. Podestá / Purification and characterization of an NAD-dependent malate dehydrogenase from leaves of the crassulacean acid metabolism plant Aptenia cordifolia / Plant Physiology and Biochemistry Volume 41, Issue 2, 1 February 2003, Pages 97-105.

DOI: 10.1016/s0981-9428(02)00015-3

[5] Kudiyarova Zh.S., Gilmanov M.K., Rakhmetova Zh.K., Omirbekova N. Zh., Bekbaeva L.K., Kurmanov B.K. The structure and functions of evolutionary young enzyme complex MDh- GOAT / Nitrogen 2007 An international symposium on the nitrogen nutrition of plants, Lancaster University- UK, 27- 31 July, (2007).

[6] L. T. Graham and M. H. Aprison / Fluorometric determination of aspartate, glutamate, and γ-aminobutyrate in nerve tissue using enzymic method /Analytical Biochemistry Volume 15, Issue 3, June 1966, Pages 487-497.

DOI: 10.1016/0003-2697(66)90110-2

[7] Osawa Y. Glutamate perception, soup stock, and the concept of umami: the ethnography, food ecology, and history of dashi in Japan. Ecol Food Nutr. 2012; 51(4): 329-45. doi: 10. 1080/03670244. 2012. 691389.

DOI: 10.1080/03670244.2012.691389