Measurement of Trap Stiffness of Holographic Optical Tweezers with Power Spectrum Method

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Abstract:

A microsphere trapped by optical tweezers moves according to the Brownian motion law, which can be described by the Langevin equation. Based on it, a quadrant photodiode (QD) is used to track the displacement of the microsphere with a diameter of 2.5um trapped by holographic optical tweezers, and power spectrum method is adopted to obtain radial trap stiffness. Experiments show that the trap stiffness increases with the increase of the laser power, and decreases as the distance between the optical trap and the inside bottom surface of the sample cell increases.

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423-426

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September 2013

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© 2013 Trans Tech Publications Ltd. All Rights Reserved

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