Analysis of the Bacterial Community by 454 Pyrosequencing in Two Freshwater Lakes: Yangcheng Lake and Taihu Lake in China

Abstract:

Have a good understanding of the bacterial community structure, especially the cyanobacteria composition, works for clarifying the overall process of harmful algae bloom in freshwater lakes. In this study, the 454 pyrosequencing method was used to investigate the bacterial community in two typical freshwater lakes of China: Yangcheng Lake and Taihu Lake. A total of 30,663 16S rRNA reads were generated from 9 samples and assigned to 3,547 operational taxonomic units (OTUs) which represented 18 recognized phyla. For Yangcheng Lake, the dominant species in both July and early September were Proteobacteria, Actinobacteria, Bacteroidetes and Cyanobacteria. Taihu Lake had the same top three dominant phyla, but with smaller proportion of cyanobacteria. With regard to genus level of cyanobacteria, there were 5 genera which contained toxin-producing ones were identified in Yangcheng Lake, which manifested higher risk for long-term harmful algae injury. The constant level of cyanobacteria proportion from pre-bloom period to forthcoming period indicated that the proliferation of cyanobacteria quantity is more a sudden process not a gradual growth pattern. Only 3 genera were found in Taihu Lake and higher background amount of Microcystis in May of Taihu Lake made it more liable to suffer harmful algae bloom.

• Periodical: Advanced Materials Research (Volume 788)
• Pages: 315-320
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.788.315
• Online since: September 2013
• Authors: Fei Bai, Si Yu Zeng, Dan Li, Yuan Li, Miao He
• Keywords: 454 Pyrosequencing, Bacterial Community, Cyanobacteria, Freshwater Lakes, Harmful Algal Bloom

References

[1] J.M. Jacoby, D.C. Collier, E.B. Welch, F.J. Hardy, M. Crayton. Environmental factors associated with a toxic bloom of Microcystis aeruginosa. Canadian Journal of Fisheries and Aquatic Sciences. Vol. 57 (2000): pp.231-240.

DOI: 10.1139/f99-234

[2] E.M. Jochimsen, W.W. Carmichael, J.S. An, D.M. Cardo, S.T. Cookson, C.E. Holmes, M.B. Antunes, D.A. de Melo Filho, T.M. Lyra, V.S. Barreto, S.M. Azevedo, W.R. Jarvis. Liver failure and death after exposure to microcystins at a hemodialysis center in Brazil. The New England Journal of Medicine, Vol. 338 (1998).

DOI: 10.1056/nejm199803263381304

[3] I.R. Falconer. An overview of problems caused by toxic blue-green algae (cyanobacteria) in drinking and recreational water. Environmental Toxicology, Vol. 14 (1999): pp.5-12.

DOI: 10.1002/(sici)1522-7278(199902)14:1<5::aid-tox3>3.0.co;2-0

[4] B. Bostroëm, A.K. Pettersson, I. Ahlgren. Seasonal dynamics of a cyanobacteria-dominated microbial community in surface sediments of a shallow eutrophic lake. Aquatic Sciences, Vol. 51 (1989): pp.153-178.

DOI: 10.1007/bf00879300

[5] H.D. Park, Y. Sasaki, T. Maruyama, E. Yanagisawa, A. Hiraishi, K. Kato. Degradation of cyanobacterial hepatotoxin microcystin by a new bacterium isolated from a hypertrophic lake. Environmental Toxicology, Vol. 16 (2001): pp.337-343.

DOI: 10.1002/tox.1041

[6] T. Saito, K. Okana, H.D. Park, T. Itayama, Y. Inamori, B.A. Neilan, B.P. Burns, N. Sugiura. Detection and sequencing of the microcystin LR degrading gene, mlrA, from new bacteria isolated from Japanese lakes. FEMS Microbiology Letters, Vol. 229 (2003).

DOI: 10.1016/s0378-1097(03)00847-4

[7] P. Qian, Y. Wang, O. Lee, S. Lau, J. Yang, F. Lafi, A. Al-Suwailem, T.Y. Wong. Vertical stratification of microbial communities in the Red Sea revealed by 16S rDNA pyrosequencing. ISME Journal, Vol. 5 (2011): pp.507-518.

DOI: 10.1038/ismej.2010.112

[8] V. Acosta-Martínez, S.E. Dowd, Y. Sun, D. Wester, V. Allen. Pyrosequencing analysis for characterization of soil bacterial populations as affected by an integrated livestock-cotton production system. Applied Soil Ecology, Vol. 45 (2010): pp.13-25.

DOI: 10.1016/j.apsoil.2010.01.005

[9] J.X. Ma, Z.W. Wang, Y. Yang, X.J. Mei, Z.C. Wu. Correlating microbial community structure and composition with aeration intensity in submerged membrane bioreactors by 454 high-throughput pyrosequencing. Water research, Vol. 47 (2013): 859-869.

DOI: 10.1016/j.watres.2012.11.013

[10] M.L. Zhang, W.J. Liu, X.B. Nie, C.P. Li, J.N. Gu, C. Zhang. Molecular analysis of bacterial communities in biofilms of a drinking water clearwell. Microbes and Environments, Vol. 27 (2012): pp.443-448.

DOI: 10.1264/jsme2.me12035

[11] T.G. Otten, H. Xu, B. Qin, G. Zhu, H.W. Paerl. Spatiotemporal Patterns and Ecophysiology of Toxigenic Microcystis Blooms in Lake Taihu, China: Implications for Water Quality Management. Environmental Science and Technology, Vol. 46 (2012).

DOI: 10.1021/es2041288

[12] R. Sharmeen and S.J. Md. Abu. Cyanobacterial blooms and water quality in two urban fish ponds. University Journal of Zoology, Rajshahi University, Vol. 27(2008): pp.79-84.

DOI: 10.3329/ujzru.v27i0.1960