Colorimetric Detection of Melamine Based on Poly-Thymine Stabilized Gold Nanoparticles

Chong Jin; Hui Min Zhao; Meng Liu; Jun Ping Tian; Xie Quan

doi:10.4028/www.scientific.net/AMR.790.619

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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 790Colorimetric Detection of Melamine Based on...

Colorimetric Detection of Melamine Based on Poly-Thymine Stabilized Gold Nanoparticles

Abstract:

A simple, fast and reliable colorimetric method for melamine detection based on gold nanoparticles (AuNPs) and poly-thymine (poly-T) is reported. Poly-T strands attract AuNPs through static electricity and keep them stable against aggregation in the presence of NaCl. The affinity between melamine and poly-T, hydrogen bond, frees AuNP from the protection provided by poly-T and thus makes AuNPs aggregate in the presence of NaCl. The color change of AuNPs from wine red to blue can be observed immediately by the naked eye during the aggregation process, and can be accurately recorded with the help of a UV/vis spetrophotometer within 5 minutes. The limit of detection (LOD) of melamine in pure water is 0.146 μmol/L with a linear range from 0.2×10^-7 mol/L to 10.0×10^-7 mol/L. In fresh milk and milk powder, the LOD is respectively as low as 2.37 μmol/L and 5.36 μmol/L.

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Periodical:

Advanced Materials Research (Volume 790)

Pages:

619-622

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.790.619

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Online since:

September 2013

Authors:

Chong Jin, Hui Min Zhao, Meng Liu, Jun Ping Tian, Xie Quan

Keywords:

Colorimetry, Gold Nanoparticles (GNP), Melamine, Poly-Thymine

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