Expression, Purification and Preparation of Polyclonal Antibody of Bombyx mori Serpin-6

Ming Hui Wang; Kai-Zun Xu; Guo Sheng Li; Yan Yun Wang; Wei De Shen; Ya Xiang Xu

doi:10.4028/www.scientific.net/AMR.796.72

Paper Titles

Cloning and Sequence Analysis of Hippo Pathway Related Major Genes of Silkworm (Bombyx mori)
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Analysis of Cocoon and Silk Quality of Trimolter Silkworms Induced by Anti-Juvenile Hormone
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Analysis of Differential Expression Proteins from Different Parts of Pistillate Flower in Mulberry (Morus alba)
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Differential Expression of Stage-Specific Fat Body Proteins during Larval-Pupal Period in Silkworm (Bombyx mori)
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Expression, Purification and Preparation of Polyclonal Antibody of Bombyx mori Serpin-6
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Observations of Skin Tissue Regenerations in Biomaterial Scaffolds with Different Biodegradability
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Recombinant Cloning of Gene Sequence Encoding Silk Fibroin Heavy Chain
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Fabrication and Characterization of Alginate Fibers by Wet-Spinning
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Preparation and Application of Low Molecular Weight Chitosan Nanoparticle as a Textile Finishing Agent
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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 796Expression, Purification and Preparation of...

Expression, Purification and Preparation of Polyclonal Antibody of Bombyx mori Serpin-6

Abstract:

Serpin (Serine Protease Inhibitor) widely distributes in animals, plants, protozoan, prokaryotes and viruses. Serpin-6 belongs to the superfamily of serine protease inhibitor. In this study, we constructed a prokaryotic vector - pET28a-serpin-6, and used IPTG (isopropyl thiogalactoside) to induce serpin-6 protein expression, then applied the Ni-affinity chromatography to purify the collected recombinant protein. The assay of SDS-PAGE and Anti-his Polyclonal antibody Test showed that the recombinant serpin-6 protein have a molecular weight of 45 kDa. The highly purified sample of the recombinant protein was obtained. The protein has been used as antigen to immunize KunMing mice using the 4 times immunization method. We successfully attained the polyclonal antibody of serpin-6.The titer of the antibody is as high as 1:20000, with a good specificity. The polyclonal antibody can provide a practical tool to further study the distribution of protein expression and functions of serpin-6 in different states and breeds of Bombyx mori.