Recombinant Cloning of Gene Sequence Encoding Silk Fibroin Heavy Chain

Zhi Fang Tian; Huan Rong Zhao; Hong Gen Yi; Jian Nan Wang

doi:10.4028/www.scientific.net/AMR.796.83

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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 796Recombinant Cloning of Gene Sequence Encoding Silk...

Recombinant Cloning of Gene Sequence Encoding Silk Fibroin Heavy Chain

Abstract:

A block combination genes (gx16-f) was designed and cloned for encoding GX16-F, which was derived from the crystalline domain (X: A, S, V or Y, GA: GAGAGA, GS: GAGAGS, GV: GAGAGV and GY: GAGAGY) and the amorphous domain (F=SGFGPVANGGSGEASSESDFGSSGFGPVANASSGEASSESDFAG) of Bombyx mori silk fibroin heavy chain. The combination genes were then cloned into a GST-tagged prokaryotic expression vector for expression of protein. Agarose gel electrophoresis analysis and DNA sequencing demonstrated that the combination gene encoding GX16-F was accurately cloned, and inserted into the expression vector successfully. The study would provide a technology to produce different structural polypeptides for studying the structurefunction relationships of silk fibroin.

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Periodical:

Advanced Materials Research (Volume 796)

Pages:

83-86

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.796.83

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Online since:

September 2013

Authors:

Zhi Fang Tian, Huan Rong Zhao, Hong Gen Yi, Jian Nan Wang

Keywords:

Gene Recombination, Heavy Chain, Silk Fibroin

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References

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