Optimization of Enzyme-Assisted Lycopene Extraction from Tomato Processing Waste

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We have investigated the recovery of lycopene from tomato processing waste (TPW) by treating the peel fraction of the waste with pectinolytic and cellulolytic enzyme preparations. To optimize the enzyme-assisted extraction, we used a two-level factorial design with five factors: extraction temperature (T), pretreatment time (P), extraction time (E), enzyme solution-to-solid ratio (R), enzyme load (L). A 7-to 16-fold increase was observed in lycopene recovery, compared to the untreated TPW. From a response surface analysis of the data, a second-degree polynomial equation was developed which provided the following optimal extraction conditions: T = 30 °C, P = 3.5 h, E = 3.2 h, R = 30 dm3/kg, L = 0.17 kg/kg.

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173-176

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September 2013

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© 2013 Trans Tech Publications Ltd. All Rights Reserved

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