Detection of 9α-OH-AD Prepared by Biotransformation

Li Min Ma; Li Cui; Yu Hang Zhao

doi:10.4028/www.scientific.net/AMR.821-822.1005

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Detection of 9α-OH-AD Prepared by Biotransformation

Abstract:

Detection of 9а-OH-AD prepared by biotransformation by RP-HPLC directly was studied.The detection is performed on a Kromasil 100-5C18(4.6×250mm) column, using methanol:water(7:3,v/v)as mobile phase,0.8mL•min^-1flow rate and external standard method,deteced at 242nm.There is a good line correlaction between peak and content in range of 0.01-0.20g/L,the correlation coefficient is 0.9942,the average recovery is 99.09% with a relative stand deviation of 0.89%(n=5).The method is simple,stable,accurate and reliable for quality control of 9а-OH-AD.

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Periodical:

Advanced Materials Research (Volumes 821-822)

Pages:

1005-1008

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.821-822.1005

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Online since:

September 2013

Authors:

Li Min Ma, Li Cui, Yu Hang Zhao

Keywords:

9а-OH-AD, Biotransformation, Quantitative Analysis, RP-HPLC

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