Staphylococcus equorum Isolated from Seabed as Potential Biotool to Cr(VI) Remediation

Abstract:

Normal 0 21 false false false ES-CL X-NONE X-NONE MicrosoftInternetExplorer4 Cr(VI) is frequently used in several industrial activities, due to its uncontrolled spill this contaminant has generated serious damage to the environment: chromium hexavalent has a high oxidation potential and is able to go through biological membranes. As consequence Cr(VI)is described as a persistent contaminant with a high toxicity. It has been searched for alternative methods to remediate or detoxify environments contaminated with Cr(VI). The use of microorganisms able to absorb or reduce this type of contaminant is discerned as a potential biotool for remediation of metals-enriched industrial wastes. In order to isolate and identify environmental microorganisms with this capacity, we isolated a bacterium named LMA-2 with a selective pressure of 60 mM of Cr(VI) from contaminated marine sediment. In the morphologic analysis, this marine microorganism evidenced that corresponds to a Gram-positive cocci. This resistant bacterium has a minimal inhibitory concentration for Cr(VI) of 750 mM. The characterization and identification of this strain was made through 16S ribosomal RNA gene, this result revealed that it corresponds to Staphylococcus equorum. Assays using atomic absorption spectrophotometer (AAS) shows that this strain is able to ireduce the concentration of soluble Cr(VI) The capacity of this strain to remediate Cr(VI) was determined in 142.8 mg/L at 10 h of exposition. Our results showed the high resistance and the ability for Cr(VI) remediation by Staphylococcus equorum. These results suggest that this marine bacterium could be a prospect for future use as a biofilter for seabed marine environment decontamination. st1\:*{behavior:url(#ieooui) } /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabla normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:10.0pt; mso-para-margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin;}

• Periodical: Advanced Materials Research (Volume 825)
• Pages: 524-527
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.825.524
• Online since: October 2013
• Authors: Paulina Pradel, Carolina Hernández, Mario Tello, Maria Paz Villarroel, Gino Corsini, Alex González
• Keywords: Bioremediation, Cr(VI), Resistant Bacteria

References

[1] J. Barnhart, Occurrences, uses and properties of chromium, Regul. Toxicol. Pharmacology. 26 (1997) S3–S7.

DOI: 10.1006/rtph.1997.1132

[2] C. Cervantes J. Campos-Garcia, Reduction and efflux of chromate by bacteria. In: Molecular Microbiology of HeavyMetals, Springer-Verlag, Berlin. (2007) Vol 37: 407- 420 pp.

[3] Environmental Protection Agency (EPA), Treatment Technologies for site cleanup: annual status report, 11 th edition, (2004).

[4] S. Sultan, S. Hasnain, Reduction of toxic hexavalent chromium by Ochrobactrum intermedium strain SDCr-5 stimulated by heavy metals, Bioresour. Technol. 98 (2007) 340–344.

DOI: 10.1016/j.biortech.2005.12.025

[5] C. González, Cuantificación de Metales Pesados en Mytilus chilensis y Sedimentos Asociados a Actividades Industriales en el Canal Tenglo, Puerto Montt. Tesis, Ingeniería en Medio Ambiente y Manejo Costero. Universidad de los Lagos, Puerto Montt, (2007).

DOI: 10.7764/tesisuc/arq/64816

[6] T. Hauschild, S. Stepanović, Identification of Staphylococcus sp. by PCR-Restriction Fragment Length Polymorphism Analysis of dnaJ Gene. J. Clin Microb. (2008) 3875.

DOI: 10.1128/jcm.00810-08

[7] K. Lee, D., Bai, Y. Smith, D. Han and H.S. Supanjani. Isolation of Plant-Growth-Promoting Endophytic Bacteria from Bean Nodules. Journal of Agriculture and Biological Sciences. Canada. (2005) 232-236 pp.

[8] T. Hauschild and S. Stepanović. Identification of Staphylococcus sp. by PCR-Restriction Fragment Length Polymorphism Analysis of dnaJ Gene. Journal of Clinical Microbiology. (2008). 3875 pp.

DOI: 10.1128/jcm.00810-08

[9] K. Mistry, C. Desai, S. Lal, K. Patel, B. Patel, Hexavalent Chromium Reduction by Staphylococcus Sp. Isolated From Cr (VI) Contaminated Land Fill, Biotechnology and Biochemistry, (2010) Vol 6: 117-129.

[10] C. Ramesh, M. Seidu, B. Michael, M. Mallavarapu, N. Ravi, Molecular characterization of chromium (VI) reducing potential in Gram positive bacteria isolated from contaminated sites, Soil Biology & Biochemistry, India. 42 (2010) 1857-1863.

DOI: 10.1016/j.soilbio.2010.07.005

[11] A. Díaz-Magaña, E. Aguilar-Barajas, R. Moreno-Sánchez, M. I. Ramírez-Díaz, H. Riveros-Rosas, E. Vargas, C. Cervantes, Short-Chain Chromate Ion Transporter Proteins from Bacillus subtilis Confer Chromate Resistance in Escherichia coli. J Bacteriol. 191 (2009).

DOI: 10.1128/jb.00625-09

[12] A. Rajbanshi. Study on Heavy Metal Resistant Bacteria in Guheswori Sewage Treatment Plant. J. Our Nature. Nepal. (2008) 6: 52-57.

DOI: 10.3126/on.v6i1.1655

[13] G. Gadd. Fungi and yeast for metal accumulation. In: Microbial Mineral Recovery, Ed. H. Ehrlich & C. L. Briely. McGraw-Hill, New York, (1990) 249-245.