Enrichment and Isolation of Phosphorus Solubilizing Bacteria

Abstract:

The aim of this study was to enrich phosphorus solubilizing microorganisms from high-phosphorus iron ores, apatite ores and phosphogypsum waste. Phosphorus solubilizing microorganisms can be utilized in dephosphorization of high-phosphorus iron ores and in phosphorus leaching from fluorapatite ores. Low grade fluorapatite ore (3.6% P, pH 6.8), fluorapatite concentrate (13% P, pH 8.3), phosphogypsum waste (0.7% P, pH 2.3), iron ore 1 (0.19% P, pH 7.6) and iron ore 2 (0.18% P, pH 7.6) were used as potential sources of phosphorus solubilizing microorganisms. The samples were cultured in NBRIP media at pH 5 and 8 with either glucose or sucrose as a carbon source, and in modified 9K media at pH 1.5 and 2.5 for 3 weeks. Phosphorus solubilizing bacteria were enriched only from the fluorapatite concentrate at the pH of 8. The four obtained heterotrophic isolates were identified by 16S rRNA gene sequencing, and were shown to be closest related to Burkholderia fungorum. These results indicate that the diversity of culturable phosphorus solubilizing bacteria present in apatite and iron ores is relatively low. The isolated Burkholderia strain showed phosphorus solubilizing potential.

• Periodical: Advanced Materials Research (Volume 825)
• Pages: 62-65
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.825.62
• Online since: October 2013
• Authors: Outi K. Priha, Tuija H. Sarlin, Mona E. Arnold, Päivi Kinnunen
• Keywords: Bioleaching, Burkholderia, Iron Ores, Phosphorus

References

[1] C.Y. Cheng, V.N. Misra, J. Clough, R. Mun: Dephosphorisation of Western Australian iron ore by hydrometallurgical process. Min Eng 12 (1999) 1083–1092.

DOI: 10.1016/s0892-6875(99)00093-x

[2] R. Chi, C. Xiao, H. Gao: Bioleaching of phosphorus from rock phosphate containing pyrites by Acidithiobacillus ferrooxidans. Min Eng 19 (2006) 979–981.

DOI: 10.1016/j.mineng.2005.10.003

[3] C. Xiao, R. Chi, W. Li, Y. Zheng: Biosolubilisation of phosphorus from rock phosphate by moderately thermophilic and mesophilic bacteria. Min Eng 24 (2011) 956–958.

DOI: 10.1016/j.mineng.2011.01.008

[4] M. Ghabaraghi, M. Irannajad, M. Noaparast: A review of the beneficiation of calcareous phosphate ores using organic acid leaching. Hydrometall 103 (2010) 96–107.

DOI: 10.1016/j.hydromet.2010.03.002

[5] C.S. Nautiyal: An efficient microbiological growth medium for screening phosphate solubilizing microorganisms. FEMS Microbiol Lett 170 (1999) 265–270.

DOI: 10.1111/j.1574-6968.1999.tb13383.x

[6] K. Katina, A. Laitila, R. Juvonen, K. -H. Liukkonen, S. Kariluoto, V. Piironen, R. Landberg, P. Åman, K. Poutanen: Bran fermentation as a means to enhance technological properties and bioactivity of rye. Food Microbiol 24 (2007) 175–186.

DOI: 10.1016/j.fm.2006.07.012

[7] C. Camacho, G. Coulouris, V. Avagyan, N. Ma, J. Papadopoulos, K. Bealer, T.L. Madden: BLAST+: architecture and applications. BMC Bioinf 10 (2009) 421.

DOI: 10.1186/1471-2105-10-421

[8] C. Lepleux, M.P. Turpault, P. Oger, P. Frey-Klett, S. Uroz: Correlation of the abundance of betaproteobacteria on mineral surfaces with mineral weathering in forest soils. Appl Env Microbiol 78 (2012) 7114–7119.

DOI: 10.1128/aem.00996-12

[9] P. Delvasto, A. Valverde, A. Ballester, J. Munoz, F. González, M. Blázquez, J. Igual, C. García-Balboa: Diversity and activity of phosphate bioleaching bacteria from a high-phosphorus iron ore. Hydrometall 92 (2008) 124–129.

DOI: 10.1016/j.hydromet.2008.02.007

[10] P. Delvasto, A. Ballester, J. Munoz, F. González, M. Blázquez, J. Igual, A. Valverde, C. García-Balboa: Mobilization of phosphorus from iron ore by the bacterium Burkholderia caribensis FeGL03. Min Eng 22 (2009) 1–9.

DOI: 10.1016/j.mineng.2008.03.001