Determination of Hexachlorocyclohexane (HCH) in Panax notoginseng of Chinese Traditional Medicine by Gas Chromatography/Mass Spectrometry

Abstract:

A novel method was developed for the determination of HCH in Panax Notoginseng of Chinese traditional medicine by ultrasonic extraction (UE) coupled with Gel Permeation Chromatography and Gas Chromatography/Mass spectrometry. Some important parameters that influence the extraction and purification efficiency were investigated. The optimum condition Panax Notoginseng of Chinese traditional medicine was extracted with dichloromethane about 20mL, extracting times and extracting time were one and 30min. The extracts were cleaned up by Gel Permeation Chromatography, and determined by Gas Chromatography /Mass spectrometry. Under the optimum condition, the mean recoveries of the method were 83.23%, the relative standard deviations (RSD) were 3.45%, which has been under the demand of the determination of HCH in Panax Notoginseng of Chinese traditional medicine.

• Periodical: Advanced Materials Research (Volume 830)
• Pages: 422-425
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.830.422
• Online since: October 2013
• Authors: Na Wu, Bo Zhou, He Ping Yan, Shi Juan Xu, Yun Hui Long, Wei Liu
• Keywords: Gas Chromatography-Mass Spectrometry (GC-MS), Gel Permeation Chromatography, HCH, Panax notoginseng, Ultrasonic Extraction

References

[1] D. Lu, Y. Yang and X. F Luo: Anal. Methods Vol. 5 (2013), P. 1721-1732.

[2] Z. Knezevic and M. Serdar: Food Control Vol. 20 (2009), P. 419.

[3] H. Rodriguez and E. Bustos-Obregon: Andrologia. Vol. 32 (1998), P. 1.

[4] M. Farghaly and S. El-Maghraby: Food Chem. Toxicol. Vol. 46 (2008), P. 3111.

[5] X.Z. Li, T.T. Guan and C. Zhou: Chem. Res. Chin. Vol. 22 (2006), P. 21.

[6] Y. Bai, L. Zhou and J. Wang: Food Chem. Vol. 98 (2006), P. 240.

[7] J. Font, A. Marsal: J. Chromatogr. A Vol. 811 (1998), P. 256.

[8] S. Vollmth, A. Zajc and R. Niessner: Environ Sci. Technol. Vol. 28 (1994), P. 1145.

[9] J. Manes, G. Font and Y. Pico: J. Chromatogr. Vol. 642 (1993), P. 195.

[10] V. Seidel, I. Tschernuter-Meixner and W. Linder: J. Chromatogr Vol. 642 (1993), P. 253.

[11] C.M. Lino, C.B.F. Azzolini and D.S.V. Nunes: J. Chromatogr. B Vol. 716 (1998), P. 147.

[12] E.R. Brouwer and U.A. Th. Brinkman: J. Chromatogr. A Vol. 678 (1994), P. 223.

[13] B.H. Hwang, M.L. Lee: Journal of Chromatography A Vol. 898 (2000), P. 245–256.