Selection of Tetraploid of a Yellow Flesh Mini-Watermelon Using Oryzalin

Abstract:

In order to provide parent material for small-type triploid watermelon, this study has been carried out to induce high-quality tetraploid watermelon. We induced tetraploid plants of watermelon by treating the growing points of seedlings of a small-type yellow flesh diploid watermelon with 40, 60 and 100ml/L Oryzalin for different treating time. The autotetraploid plants were selected and identified basing on morphology, determination of the number of chloroplasts in stomatal guard cells, flow cytometry analysis and hybridization. Then we observed a stable autotetraploid material. The results showed that tetraploid watermelons could be obtained under different treatments. The most effective way was extirpating the interior leaves at the 8th day after sowing, then treating the growing points of seedlings with 100mg/L Oryzalin for 6d. The treated seedlings could all survive and the induction frequency of the tetraploid watermelon was 33.33%.

• Periodical: Advanced Materials Research (Volumes 838-841)
• Pages: 2449-2454
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.838-841.2449
• Online since: November 2013
• Authors: Na Zhang, Hong Xia Zeng, Xian Feng Shi, Jian Ren, Wei Shun Cheng, Yong Xue Yang, Yu Hua Li, Yu Hong Sun*
• Keywords: Oryzalin, Tetraploid, Yellow Flesh Mini-Watermelon

References

[1] Michael E. Compton, D.J. Gray, Victor P, Gaba. Use of tissue culture and biotechnology for the genetic improvement of watermelon. Plant Cell, Tissue and Organ Culture, 77: 231–243( 2004).

DOI: 10.1023/b:ticu.0000018428.43446.58

[2] Liu Wen ge. Status and Development Countermeasure of Seedless Watermelon Industry in China. Journal of Changjiang Vegetables, 8: 121-127(2010).

[3] M. J. Kermani, V. arasan, A.V. Roberts. ORYZALIN-induced chromosome doubling in rosa and its effect on plant morphology and pollen viability. TheorAppl Genet, 107: 1195-1200(2003).

DOI: 10.1007/s00122-003-1374-1

[4] Leire Barandalla, Enrique Riaer. ORYZALIN treatment of potato diploids yields tetraploid and chimeric plants from which euploids could be derived by callus Induction. Potato Research, 49: 143-154(2006).

DOI: 10.1007/s11540-006-9014-1

[5] Yuan Jian min. Study of Key Technologies in Vitro and Tetraploid Induction of Mini-watermelon. Hainan University, 5-7(2010).

[6] Tan Suying, Liu Jiwei, Huang Xiu qiang, Li Sijun. Selection of New Watermelon Variety Mimei Tetraploid,. Journal of Fruit Science, 10: 87-91(1993).

[7] Qi Bi shu, Luo Yao wu, Ren Qing. A study on the relationship between meiosis of pollen mother cells and rate of berry set in tetraploid grape. Journal of Agricultural University of Hebei, 23: 49-52(2000).

[8] Shi Xian feng, Peng Jin-guang, Wang Li ping. Identifying chromosome ploidy by the number of stomatal guard cell chloroplast in watermelon. Journal of Hunan Agricultural University (Natural Sciences), 35: 640-643(2009).

[9] Zhang Jun jie, Cheng Zhi qiang. Difference in Vitamin C Content of Differently Flesh-Coloured Fruits in Watermelon. Journal of China Capsicum, 3: 34-43(2010).

[10] Cheng Zhi qiang, Liu Wen ge, Liu Zhi min, Yan Zhi hong, Zhao Sheng jie, He Nan. Comparison of vitamin C contents in watermelon fruits with different ploidy. Journal of Fruit Science, 25: 760-763(2008).