Research of Producing Single Cell Protein with Molasses Wastewater

Abstract:

This paper is to study the optimum conditions for extracting the protein feed by using the mixed microbial fermentation. And using the single factor and orthogonal methods, the microbial content as the main indicators, investigated the effects of the combination of strain, strain ratio, the amount of bacteria, fermentation temperature, the concentration of fermentation, fermentation pH, nitrogen sources and inorganic salts and other factors on the fermentation of protein yield. The test results showed that the best fermentation process is sterilization process, the best combination of strains of Geotrichum candidum, Candida tropicalis and Candida utilis yeast, the cast of bacteria was 10%, the best molasses fermentation concentration 20g / 100 mL, and temperature 30 ° C, initial pH 5.0, appropriate amount of Yeast extract, magnesium sulfate, potassium dihydrogen phosphate and super phosphate. The Test provides a theoretical and experimental basis for Molasses wastewater utilization

• Periodical: Advanced Materials Research (Volumes 838-841)
• Pages: 2785-2790
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.838-841.2785
• Online since: November 2013
• Authors: Yi Yao, Hua Lin*, Liang Liang Tan, Shao Hong You, Liao Zhang
• Keywords: Feed Protein, Fermentation Conditions, Mixed Strain

References

[1] Weilie GUO, Qinghua GUO. Fermentation of strengthened matrix protein with cane sugar refinery waste[J]. China Feed. 1995, 22: 25－26.

[2] Lianxiang DU. Industrial Microbiology experimental techniques[M]. Tianjin: Tianjin Science and Technology Press. (1992).

[3] Xin ZHAO. Study in the optimum hydrolization condition on micro-protein in the process of dealing with molasses and alcohol wastewater[J]. China Feed. 2006(14): 37-39.

[4] Lin FENG. Study on the temperature france autolysis of beer waste yeast[J]. Resources economization and comprehensive utilization. 1995(4): 31-35.

[5] Deqing ZHOU. Microbial science tutorial. Beijing: Higher Education Press. 1996. 4.

[6] PETRY S, FURLAN S, CREPEAU M J, et al. Factors Affecting Ex-ocelluar Polysaccharide Production by Lactobacillus delbrueckii ssp. Bulgaricus Grown in a Chemically Defined Medium[J] . Appl Environ Microbiol. 2000, 66 : 3427-3431.

DOI: 10.1128/aem.66.8.3427-3431.2000

[7] Ganesh K C, Joo H S, Choi J W, et al. Purification and charact-erization of an extracellular polysaccharide from haloalkalophilic Bacillus sp. I-450[J] . Enzyme and Microbial Technology, 2004, 34 (7) : 673-681.

DOI: 10.1016/j.enzmictec.2004.03.001

[8] T. C. Bhalla,M. Joshi. Protein enrichment of apple pomace by co-culture of cellulolytic moulds and yeasts[J]. World Journal of Microbiology & Biotechnology, 1994, 10(1).

DOI: 10.1007/bf00357577

[9] H. Rahmat,R. A. Hodge,G. J. Manderson,P. L. Yu. Solid-substrate fermentation ofKloeckera apiculata andCandida utilis on apple pomace to produce an improved stock-feed[J]. World Journal of Microbiology & Biotechnology, 1995, 11(2).

DOI: 10.1007/bf00704641

[10] M. Ibrahim Rajoka M.A. Tariq Kiani, Sohail Khan M.S. Awan, Abu-Saeed Hashmi. Production of single cell protein from rice polishings using Candida utilis[J]. World Journal of Microbiology and Biotechnology, 2004, 20(3).

DOI: 10.1023/b:wibi.0000023845.96123.dd