Study on the Optimum Condition for Immobilization and Properties of Laccase on Coordinated Chitosan-Cu²⁺

Abstract:

Coordinated chitosan-Cu²⁺ as a carrier, the laccase was immobilized on it by polymeric coordination method. In this study, the optimal conditions for immobilization and properties of laccase were investigated. The optimal conditions for immobilization were: CuSO₄ (0.05 mol/L), complex time (7 h), laccase concentration (250 U/mL), immobilization time (8 h). Under this condition, the activity of immobilized laccase can reach 820 U/g. In comparison with the free laccase, the optimum pH and temperature of immobilized laccase have a little change, while the heat resistance and pH stability were improved. After the immobilized laccase was stored in the refrigerator at 4 °C for 25 days, the activity of it remained 69.5 % of the original, it illustrates the immobilized laccase has a good storage stability. The laccase immobilized with chitosan-Cu²⁺ has high activity and has potential to use in industry as a biocatalyst.

• Periodical: Advanced Materials Research (Volumes 864-867)
• Pages: 1262-1265
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.864-867.1262
• Online since: December 2013
• Authors: Bo Yang*, Xu Ming Wang
• Keywords: Chitosan-Cu²⁺, Immobilisation, Laccase, Polymeric Ligand

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