Effects of Zinc on Hepatopancreatic Cell Culture of Kuruma Prawn, Litopenaeus vannamei

Hong Wei Wang; Hai Ming Xu; Chun Long Zhao; Da Li; Hui Wang; Duan Bo Cai

doi:10.4028/www.scientific.net/AMR.864-867.151

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 864-867Effects of Zinc on Hepatopancreatic Cell Culture...

Effects of Zinc on Hepatopancreatic Cell Culture of Kuruma Prawn, Litopenaeus vannamei

Abstract:

The hepatopancreatic cell culture of the kuruma prawn, Litopenaeusvannamei, was conducted to identify the effects of zinc on cell division. The culturesystem consists of medium 199 (M 199) supplemented with 0.060 mol/L NaCl,1.011g/L glucose, 1000 UI/ml penicillin, 1000 μg/ml treptomycin, 20% heatinactivated fetal calf serum (FCS) for primary cells and 10 % for subculture cells. TheRNA/DNA ratio in cultured cells was measured. The results show that the celldivision of cultured hepatopancreas cells in L. vannamei was increased by the optimalconcentration of Zn2+, 80 μg/L.

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Periodical:

Advanced Materials Research (Volumes 864-867)

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151-154

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.864-867.151

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Online since:

December 2013

Authors:

Hong Wei Wang*, Hai Ming Xu, Chun Long Zhao, Da Li, Hui Wang, Duan Bo Cai

Keywords:

Cell Culture, Hepatopancreatic, Litopenaeus vannamei, Zn²⁺

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