Rapid Detection and Quantification of Lanthanide Ions Using High-Speed Polymerase Chain Reactions

Asuka Kikuchi; Takashi Kadono; Kazuhiro Hashimoto; Kazuya Uezu; Tomonori Kawano

doi:10.4028/www.scientific.net/AMR.875-877.749

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 875-877Rapid Detection and Quantification of Lanthanide...

Rapid Detection and Quantification of Lanthanide Ions Using High-Speed Polymerase Chain Reactions

Abstract:

Recently, a novel approach to a highly sensitive and quantitative detection of rare earth element (REE) ions including La³⁺, Eu³⁺ and Tb³⁺, by the polymerase chain reaction (PCR) technique, has been reported. The detection of REE ions is based on the catalytic nature of REE ions targeting the deoxyribonucleic acid (DNA), thus monitoring of the ions can be achieved by reading the level of intact DNA by PCR. Despite of its high sensitivity (at ppb to ppt levels), the conventional PCR-based REE detection protocol requires certain length of time (1-2 hours). In the present study, we modified the PCR-based REE detection protocols by employing the high-speed PCR, and performed the automated and rapid detection of La₃₊ in small-sized aqueous samples within 5min.

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Periodical:

Advanced Materials Research (Volumes 875-877)

Pages:

749-754

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.875-877.749

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Online since:

February 2014

Authors:

Asuka Kikuchi, Takashi Kadono, Kazuhiro Hashimoto, Kazuya Uezu, Tomonori Kawano

Keywords:

DNA, Lanthanum, PCR, REE Ion

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