Bioinformatic Analysis of Codon Usage Bias in the ompC Gene of Salmonella pullorum

Article Preview

Abstract:

The aim was to identify codon usage bias between the newly comfirmed Salmonella pullorum outer membrane protein C (ompC) gene (GenBank accession No. CP003047) and that of other 21 reference Enterobacteriaceae ,and we performed comparative analysis of the codon usage bias among different organisms by a series of bioinformatics softwares The apparent codon usage bias in the 22 ompC were indicated by the codon adaptation index (CAI), effective number of codons (ENc) and the value of G+C content at the 3rd codon position ( GC3s).The Enc-plot revealed that these outer membrane protein C genes are subject to GC compositional constraints.The correlation analysis with other four organisms implied that codon usage pattern of E.coli is similar to Salmonella pullorum OmpC .

You might also be interested in these eBooks

Info:

Periodical:

Advanced Materials Research (Volumes 884-885)

Pages:

394-400

Citation:

Online since:

January 2014

Export:

Price:

Permissions CCC:

Permissions PLS:

Сopyright:

© 2014 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

[1] Gupta S K, Majumdar S, Bhattacharya TK. Ghosh TC, Studies on the relationships between the synonymous codon usage and protein secondary structural units,J. Biochem Biophys Res Commun. Vol. 269(2000)(3): 692-6.

DOI: 10.1006/bbrc.2000.2351

Google Scholar

[2] Sharp P M, and W H Li, The rate of synonymous substitution inenterobacterial genes is inversely related to codon usage bias, MolBiol Evol, Vol. 4, pp.222-230, (1987).

DOI: 10.1093/oxfordjournals.molbev.a040443

Google Scholar

[3] Xie T, Ding D, The relationship between synonymous codon usage and protein structure, FEBS Letters, Vol. 434, (1998). 93-96.

DOI: 10.1016/s0014-5793(98)01160-0

Google Scholar

[4] Suzuki H, Brown C J, Forney L J, Top E M. Comparison of correspondence analysis methods for synonymous codon usage in bacteria. DNA Res. (2008); 15: 357–65.

DOI: 10.1093/dnares/dsn028

Google Scholar

[5] Gu W, Zhou T, Ma J, Sun X, Lu Z. The relationship between synonymous codon usage and protein structure in Escherichia coli and Homo sapiens. Biosystems. Feb (2004 )73(2): 89-97.

DOI: 10.1016/j.biosystems.2003.10.001

Google Scholar

[6] Gouy M and C Gauier. Codon usage in bacteria: correlation with gene expressivity, J . Acids Research, Vol. 10, (1982)7055-7074.

DOI: 10.1093/nar/10.22.7055

Google Scholar

[7] Wan X F, Xu D, Kleinhofs A, and J Z Zhou. Quantitative relationship between synonymous codon usage bias and GC composition across unicellular genomes, J, BMC Evolutionary Biology (2004), 4: 19.

Google Scholar

[8] Mukhopadhyay P, Basak S, Ghosh T C,Synonymous codon usage in different protein secondary structural classes of human genes: implication for increased non-randomness of GC3 rich genes towards protein stability,J . Biosci, Vol. 32(2007)5: 947-63.

DOI: 10.1007/s12038-007-0095-z

Google Scholar

[9] Lee S, Weonl S, etc. Relative Codon Adaptation Index, a Sensitive Measure of Codon Usage Bias,J. Evolutionary Bioinformatics (2010): 6 47–55.

DOI: 10.4137/ebo.s4608

Google Scholar

[10] Sharp P M, and W H Li. The codon adaptation index-a measure of directional synonymous codon usage bias, and its potential applications, Nucleic Acids Research, (1987): Vol. 15. 1281-1295.

DOI: 10.1093/nar/15.3.1281

Google Scholar

[11] Ruiz LM, Armengol G, Habeych E, Orduz S. A theoretical analysis of codon adaptation index of the Boophilus microplus bm86 gene directed to the optimization of a DNA vaccine. J Theor Biol. (2006); 239: 445–9.

DOI: 10.1016/j.jtbi.2005.08.009

Google Scholar

[12] Prejit, Rajesh Kumar Agarwal, etc. Evaluation of recombinant outer membrane protein based vaccine against Salmonella Typhimurium in birds, J. Biologicals. (2013): Vol. 41 162-8.

DOI: 10.1016/j.biologicals.2013.01.003

Google Scholar

[13] Ismael S, Constantino L M, etc. Salmonella porins induce a sustained, lifelong specific bactericidal antibody memory response,J. Immunology (2006): 117(1) 59-70.

DOI: 10.1111/j.1365-2567.2005.02263.x

Google Scholar

[14] Arockiasamy A., Murthy G S, etc. Conformational epitope mapping of OmpC, a major cell surface antigen from Samonella typhi[J],Structural Biology,(2004): 148: 22-33.

DOI: 10.1016/j.jsb.2004.03.011

Google Scholar

[15] Richa Jha, Anil Kumar, etc. Cloning, Sequencing and In Silico Analysis of Omp C of Salmonella Typhimurium,J. ISRN Veterinary Science, (2012): 105402-8.

DOI: 10.5402/2012/512848

Google Scholar

[16] Barrow1 P A, Freitas Neto O C. Pullorum disease and fowl typhoid - new thoughts on old diseases: a review, J. Avian Pathology, (2011): Vol. 40(1) 1-3.

DOI: 10.1080/03079457.2010.542575

Google Scholar

[17] Richa Jha, 1 Anil Kumar etc. Cloning, Sequencing and In Silico Analysis of Omp C of Salmonella Typhimurium.J. ISRN Veterinary ScienceVolume, Article ID 512848, 7 pages.

DOI: 10.5402/2012/512848

Google Scholar

[18] Bibb M J, Findlay P R, and M W Johnson. The relationship between base composition and codon usage in bacterial genes and its use for thesimple and reliable identification of protein-coding sequences, Gene, (1984): Vol. 30, pp.157-66.

DOI: 10.1016/0378-1119(84)90116-1

Google Scholar

[19] Ikemura T. Codon usage and tRNA content in unicellular and multicellular organisms,J. Mol Biol Evol, (1985): Vol. 2, pp.13-34.

DOI: 10.1093/oxfordjournals.molbev.a040335

Google Scholar

[20] Jia M, and Y Li. The relationship among gene expression, folding free energy and codon usage bias in Escherichia coli, FEBS Letters, (2005): Vol. 579, pp.5333-5337.

DOI: 10.1016/j.febslet.2005.08.059

Google Scholar

[21] Eyre-Walker A. Synonymous codon bias is related to gene length in Escherichia coli: selection for translational accuracy?, Mol Biol Evol, (1996): Vol. 13, pp.864-872.

DOI: 10.1093/oxfordjournals.molbev.a025646

Google Scholar

[22] Sauvage C, Bierne N, Lapegue S, and P Boudry. Single Nucleotide polymorphisms and their relationship to codon usage bias in the Pacific oyster Crassostrea gigas, Gene, (2007): Vol. 406, pp.13-22.

DOI: 10.1016/j.gene.2007.05.011

Google Scholar

[23] Kotlar D, and Y Lavner. The action of selection on codon bias in the human genome is related to frequency, complexity, and chronology of amino acids. (2006): BMC Genomics. 7: 67.

DOI: 10.1186/1471-2164-7-67

Google Scholar

[24] Jansen R, Bussemaker H J, and Gerstein M. Revisiting the codon adaptation index from a whole-genome perspective: analyzing the relationship between gene expression and codon occurrence in yeast using a variety of models. Nucleic Acids Res. (2003).

DOI: 10.1093/nar/gkg306

Google Scholar

[25] Das S, Roymondal U, Sahoo S. Analyzing gene expression from relative codon usage bias in Yeast genome: a statistical significance and biological relevance. (2009): Aug 15; 443(1-2): 121-3.

DOI: 10.1016/j.gene.2009.04.022

Google Scholar