Simultaneous Separation and Determination of Aconitine Compounds in Radix aconiti praeparata by High Performance Capillary Zone Electrophoresis

Su Ya Gao; Li Wang; Yun Zhang; Hua Li

doi:10.4028/www.scientific.net/AMR.884-885.522

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 884-885Simultaneous Separation and Determination of...

Simultaneous Separation and Determination of Aconitine Compounds in Radix aconiti praeparata by High Performance Capillary Zone Electrophoresis

Abstract:

A simple and inexpensive high performance capillary zone electrophoresis (CZE) was applied to separate and determine three alkaloids in Radix Aconiti Praeparata simultaneously. The optimized conditions for separation were obtained using 20 mmol/L borax buffer (pH 9.0) containing 2 mmol/L beta-cyclodextrin (β-CD) and 20% methanol. Online UV detection was performed at 235 nm. A voltage of 16 kV was carried at 25°C. Injection was performed at 0.5psi for 10 s. The separation and determination were satisfactory and quick.

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Periodical:

Advanced Materials Research (Volumes 884-885)

Pages:

522-525

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.884-885.522

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Online since:

January 2014

Authors:

Su Ya Gao*, Li Wang, Yun Zhang, Hua Li

Keywords:

Aconitine, Capillary Zone Electrophoresis, Hypaconitine, Mesaconitine, Radix aconiti praeparata

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* - Corresponding Author

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