Influences of the Relative Dimension of Cell and Micropipette and Compressibility of Cells on the Determination of Elastic Properties of Chondrocytes during Micropipette Aspiration

Abstract:

In this study, the elastic parameters of rabbit chondrocytes were determined from a sphere model (SM) of micropipette aspiration, in which the relative geometry of cell-micropipette and compressibility of cells were considered. Further, the influences of geometry parameter ξ (ξ = R/a, R is the radius of the cell, and a is the inner radius of the micropipette) and Poissons ratio ν of cell on the determination of elastic modulus E and bulk modulus K were evaluated. The results indicated that ξ has marked effect on the calculation of E, whereas ν varying from 0.2~0.4 is little influential on E. However, the variations of ν can cause abrupt changes of K, which may affect the subsequently viscoelastic analysis significantly. This study may be of use for more precise analysis of mechanical behaviors of cells.

• Periodical: Advanced Materials Research (Volume 900)
• Pages: 279-284
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.900.279
• Online since: February 2014
• Authors: Yong Sheng Li, Quan You Zhang, Wei Yi Chen*
• Keywords: Chondrocytes, Compressibility, Micropipette Aspiration, Sphere Model

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