Research on Food Manufacturing with S-Layer Protein of Selected Lactobacillus is Responsible for Competitive Exclusion against Human Enteropathogens

Abstract:

In this study, five selected Lactobacillus of fecal origin were investigated for their probiotic properties. They were preliminarily identified by partial 16S rDNA gene sequencing and characterized in vitro for their resistance to low pH and bile salts. Here, we assessed the adhesive properties and the ability to inhibit the adhesion and to displace pathogens of five selected Lactobacillus strains using HT-29 cells model through a process which may be related to specific components of the baterial surface. Surface-layer proteins are located in a paracrystalline layer outside the bacterial cell wall and are thought to play a role in tissue adherence. Removal of S-layer proteins from the Lactobacillus (treated with 5 M LiCl) reduced adhesion to Caco-2 cells. Under such conditions, inhibition activity was also decreased in all five strains, as revealed in competition assays, which suggested that S-layer protein had involved in the adhesion of probiotics. SDS-PAGE analysis confirmed the presence of S-layer proteins with dominant bands of was approximately 60 kDa. Our findings also indicate that the surface layer proteins contributed to its increased adhesion to the cultured cells and competitive exclusion of pathogens.

• Periodical: Advanced Materials Research (Volume 910)
• Pages: 141-144
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.910.141
• Online since: March 2014
• Authors: Chao Hui Xue, Lan Wei Zhang, Hong Bo Li, Shu Mei Wang
• Keywords: Lactobacillus, Probiotic Property, Surface-Layer Proteins, Transit Tolerance

References

[1] Bhunia, A.K., Johnson, M.C. and Ray B, Direct detection of an antimicrobial peptide of Pediococcus acidilactici in sodium dodecyl sulfate-polyacrylamide gel electrophoresis, J Ind Microbiol Biot 2：319-322(1987).

DOI: 10.1007/bf01569434

[2] Batdorj, B., Dalgalarrondo, M., Choiset, Y., Pedroche, J., Métro, F. and Prévost, H, Purification and characterization of two bacteriocins produced by lactic acid bacteria isolated from Mongolian airag, J Appl Microbiol 101：837–848(2006).

DOI: 10.1111/j.1365-2672.2006.02966.x

[3] Bhunia, A. and Johnson, M. G, A modified method to directly detect in SDS–PAGE the bacteriocin of Pediococcus acidilactici, Lett Appl Microbiol 15：5–7(1992).

DOI: 10.1111/j.1472-765x.1992.tb00709.x

[4] Cheik, A., Pogori, N., Chen, H., Tian, F., Chen, W., Tang, J. and Zhang, H, Antimicrobial activity and partial characterization of bacteriocin-like inhibitory substances (BLIS) produced by Bifidobacterium infantis BCRC 14602, Food Control 20：553–559(2009).

DOI: 10.1016/j.foodcont.2008.08.003

[5] ELMER, G. W, Probiotics: "Living drugs", Am. J. Health-Syst. Pharm 58：1101-9(2001).

[6] Gilliland, S., Staley, T. and Bush, L, Importance of bile tolerance of Lactobacillus acidophilus used as dietary adjunct, J Dairy Sci 67：3045–3051( 1984).

DOI: 10.3168/jds.s0022-0302(84)81670-7

[7] Forestier, C., Champs, C.D., Vatoux, C. and Joly, B, Probiotic activities of Lactobacillus casei rhamnosus: in vitro adherence to intestinal cells and antimicrobial properties, Res Microbiol 52：167–173( 2001).

DOI: 10.1016/s0923-2508(01)01188-3

[8] Goldin, B., Gorbach, S., Saxelin, M., Barakat, S., Gualtieri, L. and Salminen, S, Survival of Lactobacillus species (Strain GG) in human gastrointestinal tract, Digest Dis Sci 37：121–128, (1992).

DOI: 10.1007/bf01308354

[9] Golowczyc, M.A., Mobili, P., Garrote, G.L., Abraham, A.G. and Antoni, G.L. D, Protective action of Lactobacillus kefir carrying S-layer protein against Salmonella enterica serovar Enteritidis, Int J of Food Microbiol 118：264–273 (2007).

DOI: 10.1016/j.ijfoodmicro.2007.07.042

[10] Lee, Y.K., Puong, K.Y., Ouwehand, A.C. and Salminen, S, Displacement of bacterial pathogens from mucus and Caco-2 cell surface by lactobacilli, J Med Microbiol 52： 925–930(2003).

DOI: 10.1099/jmm.0.05009-0

[11] Liu, Z., Jiang, Z., Zhou, K., Li, P., Liu, G. and Zhang B, Screening of bifidobacteria with acquired tolerance to human gastrointestinal tract, Anaerobe 13：215–219 (2007).

DOI: 10.1016/j.anaerobe.2007.05.002

[12] Makras, L., Triantafyllou, V., Messaoudi, D.F., Adriany, T., Zoumpopoulou, G., Tsakalidou, E., Servin, A. and Vuyst, L. D, Kinetic analysis of the antibacterial activity of probiotic lactobacilli towards Salmonella enterica serovar Typhimurium reveals a role for lactic acid and other inhibitory compounds, Res Microbiol 157：241–247(2006).

DOI: 10.1016/j.resmic.2005.09.002