Study of the Protective Effect of Schizandra chinensis Polysaccharide on Oxidative Damage of Mouse Spermatozoa In Vitro

Abstract:

The aim of the study: This study was conducted to evaluate oxidative stress damage model caused by reactive oxygen species production was prepared by heating injury and protective effect of SCP on spermatozoa membrane structure and function in vitro. Materials and methods: mouse eupyrene sperm suspensions were prepared and were randomly divided into six groups. The control group was maintained with PBS. The positive medicine group was received PBS contained vitamin E (0.25 mg /ml) .The SCP group was then given SCP (0.2, 0.4 and 0.8 mg /ml) respectively. All the groups except control group were treated with using heat stress injury. Degree of injury of spermatozoa membrane lipid peroxidation was detected. Results: The vitality of total superoxide dismutase (T-SOD) was increased and the quality of malondialdehyde (MDA) was decreased in different degree with each concentration of SCP. The protective effect of 0.4 mg /ml and 0.8 mg /ml SCP on spermatozoa membrane was obviously surpass to positive medicine group. These findings indicate that SCP could be potentially useful for the treatment of oxidative stress damage in spermatozoa.

• Periodical: Advanced Materials Research (Volumes 912-914)
• Pages: 1973-1977
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.912-914.1973
• Online since: April 2014
• Authors: Dong Hai Zhao*, Yan Zhang, Zhen Yuan, Meng Lu Li, Wei Yun Luo, Xiao Pei Han
• Keywords: Mouse Spermatozoa, Oxidative Stress Damage, Schizandra Chinensis Polysaccharide

References

[1] Yi xin WANG, Ju fen ZHENG. Male Infertility Update [J]. National Journal of Andrology, 2006, 12 (9): 771-774.

[2] Lei XU, Rui WANG, Wei xing ZHANG. Effects of Soluble Granule of Sheng Jing on Spermatogenesis Disturbance Induced by Cyclophosphamide in Mice [J]. Journal of Zheng Zhou University (Medical Sciences，2008, 43 (2): 267 -270.

[3] Rui WANG, Lei XU, Wei xing ZHANG. Sheng Jing Granule: An Effective Chinese Medicine for Spermatogenic Disturbance in Mice [J]. National Journal of Andrology, 2008, 14 (11): 1046-1049.

[4] Kai hua GUO, Fu-lu GAO, Si yun NIU. Effect of Heshouwuyin on Rb /p.53 Signal Transduction Pathway in Brain Tissue Cells of Aging Rat [J]. Acta Anatomica Sinica, 2010, 41 (3): 435-439.

[5] Ke yu XU, Jian ying XIAO. Preventive Effects of Polysaccharides from Schisandra chinensis on Liver Fibrosis in Rats [J]. China Pharmacy, 2011, 22 (19): 1743-1745.

[6] Xiao lan Huang, Ming liang Yang, Xiao min Wu. Discussion of Protective Mechanism of Lycium Barbarum Polysaccharides on Reproductive System in Rats [J]. Medical Journal of Wuhan University，2004，25 (1): 29-41.

[7] Zhi zhong YAN, Xin YANG, Cai fei DING. The Intervention Effect of Dodder on Oxidative Damage of Human Perforatorium and Ultrastructural Organization [J]. Chinese Archives of Traditional Chinese Medicine, 2006, 24 (2): 266-268.

[8] Duru NK, Morshedi M , Schu ffner A, et al. Cryopreservation-thawing of Fractionated Human Spermatozoa and Plasma Membrane Translocation of Phosphatidylserine [J]. Fertil Steril, 2001, 75 (2): 263-268.

DOI: 10.1016/s0015-0282(00)01694-0

[9] Gang LI, Wen bin ZHU, Fei NIU. The Protective Effect Research of Desertliving Cistanche Herb Phenethanol Glycoside on Oxidative Damage of Rats Spermatozoa [J]. Li Shi Zhen Medicine and Materia Medica Research 2010，21 (9): 2205-2207.