Synthesis of MCM-41 from Rice Husk Ash and its Utilization for Lipase Immobilization

Nurhadijah Zainalabidin; Noor Hasyierah Mohd Salleh; Dachyar Arbain

doi:10.4028/www.scientific.net/AMR.925.23

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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 925Synthesis of MCM-41 from Rice Husk Ash and its...

Synthesis of MCM-41 from Rice Husk Ash and its Utilization for Lipase Immobilization

Abstract:

Mesoporous materials are favorable for enzyme immobilization due to their unique properties of large surface area, uniform pore system and functionalizable surfaces. Immobilization on these materials enhances enzyme stability, activity and resistance towards harsh environmental conditions such as temperature and pH. The present study deals with synthesis of mesoporous materials namely MCM-41. For that purpose, the MCM-41 was synthesized using cetyltrimethylammonium bromide (CTAB) as a template and extractable silica from rice husk ash as a silica source. The synthesis was performed under alkaline conditions to produce white fine solid particles, followed by grafting with 3-aminopropyltriethoxysilane and activation with carbodiimide before it was immobilized with lipase. Characterization of those fine particles using Nitrogen physisorption gives BET surface area, a_{s BET}, and the pore diameterto be 1184 m²/g and 2.58 nm respectively. Fourier Transform Infrared spectra show peak of SiO₂, NH₂ and COOH functional group which confirms the existence of those compounds in covalent bonding formation between amine-grafted MCM-41 and lipase enzyme. Immobilized enzyme shows an increase in pH stability when compared to free enzyme at fixed temperature of 20°C in time range from 30 to 180 minutes.

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Periodical:

Advanced Materials Research (Volume 925)

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23-27

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.925.23

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Online since:

April 2014

Authors:

Nurhadijah Zainalabidin*, Noor Hasyierah Mohd Salleh, Dachyar Arbain

Keywords:

Enzyme Immobilization, Mesoporous Silica, Rice Husk Ash

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