Compound Injection of Shenqi as a Reversal Drug on Docetaxel Resistant Human Lung Adenocarcinoma Cell A549/DTX

Abstract:

To investigate the effect and the mechanism of compound injection of Shenqi (Codonopsis and Astragalus) on Docetaxel resistance of human lung adenocarcinoma cell A549/ DTX. The cytotoxicities of Shenqi Injection were assayed with the MTT method, the effects of Shenqi Injection on apoptosis induced by Docetaxel in A549/ DTX was examined by flow cytometry (FCM). The expression levels of apoptosis regulator Bc1-2 and Bax were detected by Western blot. After treatment with Shenqi Injection for 24 hours, results showed that 15 μL / mL Shenqi Injection significantly increased toxicity of Docetaxel on A549/DTX. Shenqi Injection could reverse the Docetaxel resistance in A549/ DTX in vitro. The drug resistance reversal index (RI) was equal to 2.71 analyzed with MTT method. Shenqi Injection could enhance the apoptosis effect of Docetaxel on A549/DTX. We conclude that Shenqi Injection can reverse the drug resistance on A549/DTX, and its mechanism is to promote apoptosis may be related with the up-regulation of Bc1-2 expression and down-regulation of Bax expression.

• Periodical: Advanced Materials Research (Volumes 926-930)
• Pages: 1054-1057
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.926-930.1054
• Online since: May 2014
• Authors: Xiao Dong Liu, Yi Ju Hou, Lin Zhang, Hui Ling Cao*
• Keywords: A549/DTX, Apoptosis, Drug Resistance, Shenqi Injection

References

[1] Martina Rudelius, Stefania Pittaluga, Satoshi Nishizuka, Blood, 2006, 108(5) : 1668-1676.

[2] Tazzari PL, Cappellini A, Ricci F, Leukemia, 2007, 21(3) : 427-433.

[3] Congcong He, Daniel J. Klionsky, Annu Rev Genet . 2009, 43: 67-93.

[4] Jendrossek V. Curr Pharm Biotechnol, 2012, 13(8)1426-38.

[5] Sotiropoulou PA, Candi A, Mascré G, et al. Nat Cell Biol, 2010, 12(6): 572-82.