3,4,5-Trihydroxybenzoic Acid Triggered Oxidative Stress in 95-D Lung Cancer Cell Line

Abstract:

It is a good therapeutic method that add exogenous ROS to trigger oxidative stress causing death of cancer cells. In the present study, we investigated the inhibitory effects of 3,4,5-trihydroxybenzoic acid (TBA), a polyhydroxyphenolic compound, on high metastatic human lung cancer cell line (95-D) based on inducing reactive oxygen species (ROS). The experiments in vitro showed that 95-D cell viability was inhibited by various amounts of TBA and death was induced in a dose-dependent manner. The possible mechanism was that TBA can induce cell death by decreasing mitochondrial membrane potential (MMP; ΔΨm) and increasing hydrogen peroxide (H₂O₂) level. These results imply that TBA efficiently induces death in 95-D lung cancer cells and that TBA exerts cytotoxicity on cancer cells by its pro-oxidative activity.

• Periodical: Advanced Materials Research (Volumes 926-930)
• Pages: 1061-1064
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.926-930.1061
• Online since: May 2014
• Authors: Yan Li Xi, Xiang Qun Wu, Jie Yu, Wei Guo Xu, Tong Zhao, Shu Ran Wang*, Feng Lan Niu, Hong Bo Ma
• Keywords: Cancer Cell Line, Hydrogen Peroxide, Oxidative Stress, Reactive Oxygen Species, TBA

References

[1] W.S. Beckett: Clin Chest Med, Vol. 14 (1993), p.1.

[2] R.K. Gill, M.F. Vazquez, A. Kramer, M. Hames, L. Zhang, K. Heselmeyer-Haddad, T. Ried, K. Shilo, C. Henschke, D. Yankelevitz and J. Jen: Clin Cancer Res, Vol. 14 (2008), p.7481.

DOI: 10.1158/1078-0432.ccr-07-5242

[3] A.F. Cheung, M.J. Dupage, H.K. Dong, J. Chen and T. Jacks: Cancer Res, Vol. 68 (2008), p.9459.

[4] D.K. Maurya, N. Nandakumar and T.P. Devasagayam: J Clin Biochem Nutr, Vol. 48 (2011), p.85.

[5] C. Gonzalez, G. Sanz-Alfayate, M.T. Agapito, A. Gomez-Nino, A. Rocher and A. Obeso: Respir Physiol Neurobiol, Vol. 132 (2002), p.17.

[6] C.P. Baran, M.M. Zeigler, S. Tridandapani and C.B. Marsh: Curr Pharm Des, Vol. 10 (2004), p.855.

[7] S.B. Wallach-Dayan, G. Izbicki, P.Y. Cohen, R. Gerstl-Golan, A. Fine and R. Breuer: Am J Physiol Lung Cell Mol Physiol, Vol. 290 (2006), p. L790.

DOI: 10.1152/ajplung.00300.2004

[8] H.U. Simon, A. Haj-Yehia and F. Levi-Schaffer: Apoptosis, Vol. 5 (2000), p.415.

[9] R. Niemetz and G.G. Gross: Phytochemistry, Vol. 66 (2005), p. (2001).

[10] E. Klein and N. Weber: J Agric Food Chem, Vol. 49 (2001), p.1224.

[11] S.K. Manna, M.T. Kuo and B.B. Aggarwal: Oncogene, Vol. 18 (1999), p.4371.

[12] S.M. Fiuza, C. Gomes, L.J. Teixeira, M.T. Girao da Cruz, M.N. Cordeiro, N. Milhazes, F. Borges and M.P. Marques: Bioorg Med Chem, Vol. 12 (2004), p.3581.

[13] A. Serrano, C. Palacios, G. Roy, C. Cespon, M.L. Villar, M. Nocito and P. Gonzalez-Porque: Arch Biochem Biophys, Vol. 350 (1998), p.49.

[14] M. Inoue, N. Sakaguchi, K. Isuzugawa, H. Tani and Y. Ogihara: Biol Pharm Bull, Vol. 23 (2000), p.1153.

[15] K. Isuzugawa, M. Inoue and Y. Ogihara: Biol Pharm Bull, Vol. 24 (2001), p.1022.

[16] K. Isuzugawa, Y. Ogihara and M. Inoue: Biol Pharm Bull, Vol. 24 (2001), p.249.

[17] S.K. Mantena, S.D. Sharma and S.K. Katiyar Berberine: Mol Cancer Ther, Vol. 5 (2006), p.296.

[18] C.A. Gomes, T.G. da Cruz, J.L. Andrade, N. Milhazes, F. Borges and M.P. Marques: J Med Chem, Vol. 46 (2003), p.5395.

[19] N. Sakaguchi, M. Inoue and Y. Ogihara: Biochem Pharmacol, Vol. 55 (1998), p. (1973).