Diversity and Distribution of Sulfate-Reducing Bacteria in Jilin Oilfield

Abstract:

To better understand the roles of microorganisms in oil production, diversity and distribution of microbes in Jilin oilfield was studied. Firstly, chromosomal DNA wassuccessfully extracted directly from crude oil samples. Diversity and distribution of microbes was then analyzed based on 16S rDNA libraries. There were totally 21 OTUs were obtained through 16S rDNA sequencing. Of those OTUs, 13 are bacteria in which Proteobacteria is the major family, while 8 are archaea in which Methanomicrobia is the dominant. Finally, SRB was found in all samples by amplifying the apsA gene using PCR. SRB found in Jilin oil samples belong to δ-Proteobacteria.

• Periodical: Advanced Materials Research (Volume 937)
• Pages: 297-302
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.937.297
• Online since: May 2014
• Authors: Wen Feng Song*, De Sheng Ma, You Yi Zhu, Xiao Fang Wei, Jie Wu
• Keywords: Distribution, Oil Reservoir, OTUs, PCR, Phylogenetic, Proteobacteria, SRB, Sulfate

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