Cultivation and Regulation of Sulfate-Reducing Bacteria in Oilfield

Abstract:

To better regulate (up and down) the activation of SRB thereby contributing to EOR, efficiency of growth and inhibition of SRB was studied in this paper.Six different growth media were tested in total.The optimal growth media were selected based on growth rate and variations of pH between the start and end point of growth. Of the six media, complex I was selected as the best medium in which SRB grew to stationary-phase in less than 8 days with the highest cells’ concentration. Finally, the effects of NO₃^- on growth and persistence of SRB were studied by using abroad range concentrations of NO₃^-. The result indicated that SRB growth could be significantly inhibited at NO₃^- of 80 mM.

• Periodical: Advanced Materials Research (Volume 937)
• Pages: 303-307
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.937.303
• Online since: May 2014
• Authors: Wen Feng Song*, De Sheng Ma, You Yi Zhu, Xiao Fang Wei, Jie Wu
• Keywords: EOR, Hydrogen Sulfide, Nitrate, Nitrite, SRB, Sulfate

References

[1] G. Muyzer and A.J.M. Stams, The ecology and biotechnology of Sulphate-reducing bacteria, Nat. Rev. Microbiol. 6 (2008) 441-454.

DOI: 10.1038/nrmicro1892

[2] S.M. Keith, R.A. Herbert, C.G. Harfoot, Isolation of new types of Sulphate-reducing bacteria from estuarine and marine sediments using chemostat enrichments, J. Appl. Bacteriol. 53 (1982) 29-33.

DOI: 10.1111/j.1365-2672.1982.tb04731.x

[3] R.J. Parkes, G.R. Gibson, I. Mueller-Harvey, W.J. Buckingham, R. Herbert, Determination of the substrates for Sulphate-reducing bacteria within marine and estuarine sediments with different rates of Sulphate reduction, J. Gen. Microbiol. 135 (1989).

DOI: 10.1099/00221287-135-1-175

[4] F. Bak and N. Pfennig, Sulfate-reducing bacteria in littoral sediment of Lake Constance, FEMS Microbiol. Ecol. 85 (1991) 43-52.

DOI: 10.1111/j.1574-6968.1991.tb04696.x

[5] P. Caumette, Ecology and physiology of phototrophic bacteria and Sulphate-reducing bacteria in marine salterns, Experientia. 49 (1993) 473-481.

DOI: 10.1007/bf01955148

[6] J.R. Postgate, TheSulphate-Reducing Bacteria, Cambridge U. Press. London (1984).

[7] F. Widdel, and N. Pfennig, Bergey's Manual of Systematic Bacteriology, Williams & Wilkins, Baltimore 1 (1984) 663-679.

[8] R. Cord-Ruwisch, W. Kleinitz, and F. Widdel, Sulfate-Reducing Bacteria and Their Activities in Oil Production, J. Pet. Technol. 39 (1987) 97-106.

DOI: 10.2118/13554-pa

[9] A. Suarez-Suarez, A. Lopez-Lopez, A. Tovar-Sanchez, P. Yarza, A. Orfila, J. Terrados, J. Arnds, S. Marques, H. Niemann, P. Schmitt-Kopplin, R. Amann, R. Rossello-Mora, Response of sulfate-reducing bacteria to an artificial oil-spill in a coastal marine sediment, Environ. Microbiol. 13 (2011).

DOI: 10.1111/j.1462-2920.2011.02451.x

[10] M. Rzeczycka, and M. Blaszczyk, Growth and activity of Sulphate-reducing bacteria in media containing phosphogypsum and different sources of carbon, Pol. Environ. Stud. 14 (2005) 891-895.

[11] G.E. Jenneman, M.J. McInerney, and R.M. Knapp, Effect of nitrate on biogenic sulfide production, Appl. Environ. Microbiol. 51 (1986) 1205-1211.

DOI: 10.1128/aem.51.6.1205-1211.1986

[12] I. Davidova, M.S. Hicks, P.M. Fedorak, J.M. Duflita, The influence of nitrate on microbial processes in oil industry production waters, J. Ind. Microbiol. Biotechnol. 27 (2001) 80-86.

DOI: 10.1038/sj.jim.7000166

[13] Q. He, Z. He, D.C. Joyner, M. Joachimiak, M.N. Price, Z.K. Yang, H.C.B. Yen, C.L. Hemme, W. Chen, M.M. Fields, D.A. Stahl, J.D. Keasling, M. Keller, A.P. Arkin, T.C. Hazen, J.D. Wall, J. Zhou, Impact of elevated nitrate on sulfate-reducing bacteria: a comparative study of Desulfovibrio vulgaris, ISME. J. 4 (2010).

DOI: 10.1038/ismej.2010.59

[14] R.J. Parkes, N.J.E. Dowling, D.C. White, R.A. Herbert, G.R. Gibson, Characterization of sulphate-reducing bacterial populations within marine and estuarine sediments with different rates of sulphate reduction, FEMS. Microbiol. Ecol. 102 (1993).

DOI: 10.1111/j.1574-6968.1993.tb05815.x

[15] D. Shao, Y. Kang, A. Wu, M.H. Wong, Effects of sulfate reducing bacteria and sulfate concentrations on mercury methylation in freshwater sediments, Sci. Total. Environ. 424 (2012) 331-336.

DOI: 10.1016/j.scitotenv.2011.09.042

[16] K.T. Finneran, M.E. Housewright, and D.R. Lovley, Multiple influences of nitrate on uranium solubility during bioremediation of uranium-contaminated subsurface sediments, Environ. Microbiol. 4 (2002) 510-516.

DOI: 10.1046/j.1462-2920.2002.00317.x

[17] J.D. Istok, J.M. Senko, L.R. Krumholz, et al. In situ bioreduction of technetium and uranium in a nitrate-contaminated aquifer, Environ. Sci. Technol. 38 (2004) 468-475.

DOI: 10.1021/es034639p

[18] J.L. Nyman, T.L. Marsh, M.A. Ginder-Vogel, M. Gentile, S. Fendorf, C. Criddle, Heterogeneous response to biostimulation for U (VI) reduction in replicated sediment microcosms, Biodegradation. 17 (2006) 303-316.

DOI: 10.1007/s10532-005-9000-3

[19] E.A. Greene, C. Hubert, M. Nemati, G.E. Jenneman, G. Voordouw, Nitrite reductase activity of sulfate-reducing bacteria prevents their inhibition by nitrate-reducing, sulfide-oxidizing bacteria, Environ. Microbiol. 5 (2003) 607-617.

DOI: 10.1046/j.1462-2920.2003.00446.x

[20] S.A. Haveman, E.A. Greene, C.P. Stilwell, J.K. Voordouw, G. Voordouw, Physiological and gene expression analysis of inhibition of Desulfovibrio vulgaris Hildenborough by nitrite, J. Bacteriol. 186 (2004) 7944-7950.

DOI: 10.1128/jb.186.23.7944-7950.2004