Synthesis of Vitamin B12 Artificial Antigen and Determination of Hapten Coupling Ratio

Fan Fan Yang; Li Xin Zhu; Ren Rong Liu; Long Xu; Meng Wei

doi:10.4028/www.scientific.net/AMR.955-959.1009

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 955-959Synthesis of Vitamin B12 Artificial Antigen and...

Synthesis of Vitamin B₁₂ Artificial Antigen and Determination of Hapten Coupling Ratio

Abstract:

Vitamin B₁₂ (vB₁₂) was coupled to cBSA by N,N'-Carbonyldiimidazole (CDI) method. The result was confirmed by UV spectrum analysis. It showed that vB₁₂ conjugates (vB12-cBSA) were successfully synthesized. By using UV scanning the coupling ratio of artificial antigen was determined which was 6:1. The successful synthesis of vitamin B₁₂ artificial antigen is important to establish immunoassay for determining vitamin B₁₂

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Periodical:

Advanced Materials Research (Volumes 955-959)

Pages:

1009-1012

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.955-959.1009

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Online since:

June 2014

Authors:

Fan Fan Yang, Li Xin Zhu, Ren Rong Liu*, Long Xu, Meng Wei

Keywords:

Artificial Antigen, N,N'-Carbonyldiimidazole Method, Vitamin B₁₂

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