Synthesis of Vitamin B12 Artificial Antigen and Determination of Hapten Coupling Ratio

Abstract:

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Vitamin B12 (vB12) was coupled to cBSA by N,N'-Carbonyldiimidazole (CDI) method. The result was confirmed by UV spectrum analysis. It showed that vB12 conjugates (vB12-cBSA) were successfully synthesized. By using UV scanning the coupling ratio of artificial antigen was determined which was 6:1. The successful synthesis of vitamin B12 artificial antigen is important to establish immunoassay for determining vitamin B12

Info:

Periodical:

Advanced Materials Research (Volumes 955-959)

Edited by:

Ji Zhao, Aijie Wang, Xinyong Li and Xiaoyi Wang

Pages:

1009-1012

Citation:

F. F. Yang et al., "Synthesis of Vitamin B12 Artificial Antigen and Determination of Hapten Coupling Ratio", Advanced Materials Research, Vols. 955-959, pp. 1009-1012, 2014

Online since:

June 2014

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$38.00

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