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Investigating the Interaction of Ninhydrin with Amino Acids and Polypeptide

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Abstract:

Ninhydrin chromogenic reactions are widely used to analyze and characterize amino acids, peptides, and proteins. In this study, the spectroscopy behavior of ninhydrin reaction with mercapto amino acid and polypeptide has been investigated. The maximum absorption wavelength of glutathione reaction with ninhydrin was 568 nm; the maximum absorption wavelength of cysteine reaction with ninhydrin was 457 nm. Under the optimized wavelength condition, glutathione and cysteine in the certain concentration range exhibt good linear relationship and low detection limit.

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Periodical:

Advanced Materials Research (Volumes 955-959)

Pages:

395-398

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.955-959.395

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Online since:

June 2014

Authors:

Shu Yuan Hu, Hui Qing Li, Lian Wei Yu, Fu Li Tian*

Keywords:

Cysteine, Glutathione, Interaction, Ninhydrin, UV-VIS Spectroscopy

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© 2014 Trans Tech Publications Ltd. All Rights Reserved

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