One-Step Purification of Catechol 2,3-dioxygenase from Bacillus cereus ZL1

Abstract:

Catechol 2,3-dioxygenase (C23O), the key enzyme catalyzing the second step in the phenol degradation meta-cleavage pathway has been purified to homogeneity by one-step chromatography from bacterial strain Bacillus cereus ZL1. The culture condition was optimized according to the analysis of the biomass and C23O activity, and the separation process was monitored by SDS-PAGE method. The molecular weight of the purified C23O was 37±3KDa and the active enzyme was eluted by potassium phosphate buffer with 400 mmol NaCL using linear gradient mode on DEAE-Sepharose Fast Flow.

• Periodical: Advanced Materials Research (Volumes 955-959)
• Pages: 455-458
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.955-959.455
• Online since: June 2014
• Authors: Lin Zhou*, Zi Xiong Zhou, Shuang Zhu
• Keywords: Biocatalysis, Catechol 2,3-dioxygenase, One-Step Purification

References

[1] T. Kobayashi, T. Ishida, K. Horiike, Y. Takahara, N. Numao, A. Nakazawa, T . Nakazawa, M. Nozaki: J Biochem Vol. 117(1995), p.614.

DOI: 10.1093/oxfordjournals.jbchem.a124753

[2] Takeo, M., M. Nishimura, et al. Biosci Biotechnol Biochem Vol. 71(2007), p.1668.

[3] M.Q. Chen, C.C. Yin, W. Zhang, Y.M. Mao, Z.H. Zhang. Acta Crystallogr D Biol Crystallogr Vol. 54(1998), p.446.

[4] A. Kita, S. Kita, I. Fujisawa, K. Inaka, T. Ishida, K. Horiike, M. Nozaki, K. Miki: Structure Vol. 7(1999), p.25.

DOI: 10.2210/pdb1mpy/pdb

[5] S. Murakami, Y. Nakanishi, N. Kodama, S. Takenaka, R. Shinke, K. Aoki: Biosci Biotechnol Biochem Vol. 62 (1998), p.747.

[6] M. Corredig, D.G. Dalgleish: Int. Dairy J, Vol. 9(1999), p.233.