Effect of Lithium on Growth Process of Environmental Microorganism by Microcalorimetry and SEM

Abstract:

Bacillus thuringiensis (BT) was the typical environmental microorganism.To explore the environmental behavior of lithium, the growth metabolism of BT under Li+ was investigated by microcalorimetry. The power-time curve showed the growth metabolic power-time curve of BT was divided into the bacterial growth phase and sporulation process. Lithium at 0.1-12mmol L-1 revealed no obvious effect to the bacterial growth phase of BT . Li+ at 0.1-4mmol L-1 stimulated the sporulation production and Li+ at 12-16mmol L-1 inhibited that respectively. While the time for initiation to produce sporulation was not influenced. However, the time for initiation to produce sporulation was earlier than the control induced by 8 mmol L-1 of Li+. The interest turning point of microcalorimetric result was confirmed by the following SEM detection. Besides the proof on environmental behavior of lithium, present results may bring related information for BT spores biological engineering transformation.

• Periodical: Advanced Materials Research (Volumes 955-959)
• Pages: 445-449
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.955-959.445
• Online since: June 2014
• Authors: Hui Rong Li, Wei Ming Liu, Shi Jing Cheng, Yang Jiang
• Keywords: Bacillus Thuringiensis, Lthium, Microcalorimetry, SEM

References

[1] H. R. Pilcher, : Nature( 2003).

[2] E. M. Knijff, C. Ruwhof, Biological Psychiatry 2006, 59 (4): 317-326.

[3] J.K. Rybakowslu: Pharmacopsychiatry2000, 33: 159-164.

[4] E. M. Kniff, R. W. Kupka and C. Ruwhof: Bipolar Disorders 2005, 7 (4): 388-389.

[5] Y.Y. Liu, G. Pluijm and M. Karperien: Clinical Endocrinology 2006, 64 (6): 617-624.

[6] E. Beurel, M. Kornprobst, M. J. B. Eggelpoel: Experimental Cell Research 2004, 300 (2): 354-364.

[7] S. S. Koong, J. C. Reynolds and E. G. Movius: Journal of Clinical Endocrinology and Metabolism 1999, 84 (3): 912-916.

[8] L.A. Kszos, A.J. Stemart: Ecotoxicology, 2003, 12: 439-447.

[9] L.A. Kszos , J.J. Beauchamp, A.J. Stewart: Ecotoxicology 2003, 12 (5): 427-437.

[10] H.R. Li, Y. Liu, C.Q. Qin: Biological Trace Element Research 2007, 119: 60-67.

[11] G.T. Vilas-Boas, M.V.F. Lemos: Canadian Journal of Microbiology 2004, 50: 605-613.

[12] M. Giovannetti, C. Sbrana, A. Turrini: Rivista Di Biologia-Biology Forum, 2005, 98 (3): 393-417.

[13] A. L. Haglund, P. Lantz, E. Tornblom: FEMS Microbiology Ecology, 2003, 46: 31-38.

[14] A. G. S. Prado, C. Airoldi: : Pest Management Science, 2001, 57 : 640-644.

[15] Nunez-Regueira L, Rodriguez-Anon, JA, Proupin-Castineiras J, Nunez-Fernandez O, Microcalorimetric study of changes in the microbial activity in a humic Cambisol after reforestation with eucalyptus in Galicia (NW Spain)[J]. Soil Biology & Biochemistry 2006, 38 (1), 115-124.

DOI: 10.1016/j.soilbio.2005.04.031

[16] Barros N, Gallego M and Feijoo S. : Calculation of the specific rate of catabolic activity (A(c) from the heat flow rate of soil microbial reactions measured by calorimetry: Significance and applications [J]. Chemistry and Biodiersity, 2004, 1: 1560-1568.

DOI: 10.1002/cbdv.200490116

[17] Kaya, A. Characterization of clay particle surfaces for contaminant sorption in soil barriers using flow microcalorimetry[J]. Journal of Environmental Engineering-ASCE 2004, 130: 918-921.

DOI: 10.1061/(asce)0733-9372(2004)130:8(918)

[18] Wadsö, I. Isothermal microcalorimetry in applied biology[J]. Thermochimica. Acta, 2002, 394: 305-311.

DOI: 10.1016/s0040-6031(02)00263-0

[19] H.R. Li, Z.J. Ku, C.Q. Qin: Chinese Journal of Chemistry, 2007, 25; 1798-1801.

[20] L. Wadso, F. Gomez, I. Sjoholm, : Thermochun Acta, 2004, 422: 89-93.

[21] A.M. Babu, T. Philip and B.K. Kariappa: Journal of Phytopatology. 2009, 157: 57-62.

[22] P. Vellanki, G. Jayaraman, I. W. Marison: Thermochimica Acta. 1998, 309 (1-2): 105-110.