Process Simulation and Economic Analysis of Rhamnolipid Production by Pseudomonas aeruginosa Using Oil Palm Empty Fruit Bunch as Substrate

Article Preview

Abstract:

Surfactants are amphiphilic molecules that have been used in widely used as emulsifier and cleaning agent in various industries. These broad industrial applications made the global surfactant production increased annually. In 2020, the amount of global surfactant production estimated would reach more than 24 million per year. However, the currently used surfactants are synthetically produced from chemical or petroleum-derived products, so it is often toxic and non-biodegradable. Rhamnolipids are “surfactant like” molecules produced by Pseudomonas aeruginosa, which could be alternatives for more environmentally friendly surfactants. The use of rhamnolipids is quite limited due to its expensive production cost. The production cost of rhamnolipid could be reduced using by agro-industrial by-product as a substrate. One of the abundant agro-industrial by-products in Indonesia that can be used as a substrate for rhamnolipid production is Oil Palm Empty Fruit Bunch (OPEFB). This study employed SuperPro Designer v9.5 to performed process simulation and economic assessment of rhamnolipid production using OPEFB as a substrate through two different purification methods, which are solvent extraction and chromatography adsorption. Based on the process simulation that has been done, the process that used adsorption chromatography purification methods more efficient in terms of the usage of the feedstock and energy. The economic assessment also showed that these methods were more profitable and economically attractive, with the value of NPV 11.400.000 USD, IRR 11,64%, and Payback Period 6,21 years.

You might also be interested in these eBooks

Info:

Periodical:

Pages:

103-112

Citation:

Online since:

February 2021

Export:

Price:

Permissions CCC:

Permissions PLS:

Сopyright:

© 2021 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

* - Corresponding Author

[1] Yu, Hui, and Guo He Huang. Isolation and characterization of biosurfactant-and bioemulsifier-producing bacteria from petroleum contaminated sites in Western Canada, Soil and Sediment Contamination 20.3 (2011) 274-288.

DOI: 10.1080/15320383.2011.560981

Google Scholar

[2] Banat, Ibrahim M., Andrea Franzetti, Isabella Gandolfi, Giuseppina Bestetti, Maria G. Martinotti, Letizia Fracchia, Thomas J. Smyth, and Roger Marchant. Microbial biosurfactants production, applications and future potential, Applied microbiology and biotechnology 87, no. 2 (2010) 427-444.

DOI: 10.1007/s00253-010-2589-0

Google Scholar

[3] Gudiña, Eduardo J., Ana I. Rodrigues, Victor de Freitas, Zélia Azevedo, José A. Teixeira, and Lígia R. Rodrigues. Valorization of agro-industrial wastes towards the production of rhamnolipids, Bioresource technology 212 (2016) 144-150.

DOI: 10.1016/j.biortech.2016.04.027

Google Scholar

[4] Reis, R.S., G.J. Pacheco, A.G. Pereira, and D.M.G. Freire, Biosurfactants: production and applications, Biodegradation-life of science, (2013) 31-61.

DOI: 10.5772/56144

Google Scholar

[5] Abdel-Mawgoud, Ahmad Mohammad, Rudolf Hausmann, Francois Lépine, Markus M. Müller, and Eric Déziel, Rhamnolipids: detection, analysis, biosynthesis, genetic regulation, and bioengineering of production, in: Biosurfactants, Springer, Berlin, Heidelberg, 2011, pp.13-55.

DOI: 10.1007/978-3-642-14490-5_2

Google Scholar

[6] Li, Qingxin, Rhamnolipid synthesis and production with diverse resources, Frontiers of Chemical Science and Engineering 11, no. 1 (2017) 27-36.

Google Scholar

[7] Abbasi, Habib, Mir Manochehr Hamedi, Tayebe Bagheri Lotfabad, Hossein Shahbani Zahiri, Hakimeh Sharafi, Fatemeh Masoomi, Ali Akbar Moosavi-Movahedi, Antonio Ortiz, Massoud Amanlou, and Kambiz Akbari Noghabi, Biosurfactant-producing bacterium, Pseudomonas aeruginosa MA01 isolated from spoiled apples: physicochemical and structural characteristics of isolated biosurfactant, Journal of bioscience and bioengineering 113, no. 2 (2012) 211-219.

DOI: 10.1016/j.jbiosc.2011.10.002

Google Scholar

[8] Silva, Rita De Cássia FS, Darne G. Almeida, Raquel D. Rufino, Juliana M. Luna, Valdemir A. Santos, and Leonie Asfora Sarubbo, Applications of biosurfactants in the petroleum industry and the remediation of oil spills, International journal of molecular sciences 15, no. 7 (2014) 12523-12542.

DOI: 10.3390/ijms150712523

Google Scholar

[9] Sinumvayo, Jean Paul, and Nestor Ishimwe., Agriculture and food applications of rhamnolipids and its production by Pseudomonas aeruginosa, Journal of Chemical Engineering & Process Technology 6, no. 2 (2015) 223.

DOI: 10.4172/2157-7048.1000223

Google Scholar

[10] Varnier, A.L., Lisa Sanchez, Parul Vatsa, Leslie Boudesocque, Angela, Garcia‐Brigger, Fanja Rabenoelina, Alexander Sorokin et al. Bacterial rhamnolipids are novel MAMPs conferring resistance to Botrytis cinerea in grapevine, Plant, cell & environment 32, no. 2 (2009) 178-193.

DOI: 10.1111/j.1365-3040.2008.01911.x

Google Scholar

[11] Sekhon Randhawa, Kamaljeet K., and Pattanathu KSM Rahman, Rhamnolipid biosurfactants—past, present, and future scenario of global market, Frontiers in microbiology 5 (2014) 454.

DOI: 10.3389/fmicb.2014.00454

Google Scholar

[12] Hrůzová, Kateřina, Alok Patel, Jan Masák, Olga Maťátková, Ulrika Rova, Paul Christakopoulos, and Leonidas Matsakas, A novel approach for the production of green biosurfactant from Pseudomonas aeruginosa using renewable forest biomass, Science of The Total Environment 711 (2020) 135099.

DOI: 10.1016/j.scitotenv.2019.135099

Google Scholar

[13] Nitschke, Marcia, Siddhartha GVAO Costa, and Jonas Contiero, Structure and applications of a rhamnolipid surfactant produced in soybean oil waste, Applied biochemistry and biotechnology 160, no. 7 (2010) 2066-2074.

DOI: 10.1007/s12010-009-8707-8

Google Scholar

[14] Benincasa, Maria, and Fábio Raphael Accorsini, Pseudomonas aeruginosa LBI production as an integrated process using the wastes from sunflower-oil refining as a substrate, Bioresource Technology 99, no. 9 (2008) 3843-3849.

DOI: 10.1016/j.biortech.2007.06.048

Google Scholar

[15] Henkel, Marius, Markus M. Müller, Johannes H. Kügler, Roberta B. Lovaglio, Jonas Contiero, Christoph Syldatk, and Rudolf Hausmann, Rhamnolipids as biosurfactants from renewable resources: concepts for next-generation rhamnolipid production, Process Biochemistry 47, no. 8 (2012) 1207-1219.

DOI: 10.1016/j.procbio.2012.04.018

Google Scholar

[16] Tan, Yun Nian, and Qingxin Li., Microbial production of rhamnolipids using sugars as carbon sources, Microbial cell factories 17, no. 1 (2018) 89.

DOI: 10.1186/s12934-018-0938-3

Google Scholar

[17] Pertanian, DJP Kementrian, Statistik Perkebunan Indonesia 2018-2020, Jakarta: Kementerian Pertanian, (2019).

Google Scholar

[18] Hambali, E., and M. Rivai., The potential of palm oil waste biomass in Indonesia in 2020 and 2030, In IOP Conference Series: Earth and Environmental Science, vol. 65, no. 1. IOP Publishing, (2017).

DOI: 10.1088/1755-1315/65/1/012050

Google Scholar

[19] Information on http://lipi.go.id/berita.

Google Scholar

[20] Surya, E.A., S.F. Rahman, S. Zulamraini, and M. Gozan., Preliminary plant design of Escherichia coli BPPTCC-EgRK2 cell culture for recombinant cellulase production using Oil Palm Empty Fruit Bunch (OPEFB) as substrate, In IOP Conference Series: Earth and Environmental Science, vol. 141, no. 1, p.012030. IOP Publishing Ltd., (2018).

DOI: 10.1088/1755-1315/141/1/012030

Google Scholar

[21] Han, Minhee, Yule Kim, Seung Wook Kim, and Gi‐Wook Choi., High efficiency bioethanol production from OPEFB using pilot pretreatment reactor, Journal of Chemical Technology & Biotechnology 86, no. 12 (2011) 1527-1534.

DOI: 10.1002/jctb.2668

Google Scholar

[22] Jeon, Hyungjin, Kyeong-Eop Kang, Jun-Seong Jeong, Gyeongtaek Gong, Jae-Wook Choi, Haznan Abimanyu, Byoung Sung Ahn, Dong-Jin Suh, and Gi-Wook Choi., Production of anhydrous ethanol using oil palm empty fruit bunch in a pilot plant, Biomass and bioenergy 67 (2014) 99-107.

DOI: 10.1016/j.biombioe.2014.04.022

Google Scholar

[23] NREL (National Renewable Energy Laboratory) in Golden, CO., Enzymatic saccharification of lignocellulosic biomass, in the LAP (laboratory analytical procedure) (2008).

DOI: 10.2172/937357

Google Scholar

[24] Tomar, G.S., and G. Srinikethan., Studies on production of biosurfactant from Pseudomonas aeruginosa (MTCC7815) & its application in microbial enhanced oil recovery, Res. J. Chem. Environ. Sci 4 (2016) 84-91.

Google Scholar

[25] Tiso, Till, Andrea Germer, Benjamin Küpper, Rolf Wichmann, and Lars M. Blank., Methods for recombinant rhamnolipid production, in: Hydrocarbon and Lipid Microbiology Protocols, Springer, Berlin, Heidelberg, 2015, pp.65-94.

DOI: 10.1007/8623_2015_60

Google Scholar

[26] Doran, P.M., Material balances, in: Bioprocess Engineering Principles Second Edition, Academic Press of Elsevier, Waltham, MA, 2013, pp.87-137.

Google Scholar

[27] Pathaka, A.N., and H. Pranav., Optimization of rhamnolipid: A new age biosurfactant from Pseudomonas aeruginosa MTCC 1688 and its application in oil recovery, heavy and toxic metals recovery, J. Bioprocess Biotech 5 (2015) 1-29.

DOI: 10.4172/2155-9821.1000229

Google Scholar

[28] Reiling, H.E., U. Thanei-Wyss, L.H. Guerra-Santos, R. Hirt, O. Käppeli, and A. Fiechter., Pilot plant production of rhamnolipid biosurfactant by Pseudomonas aeruginosa, Applied and Environmental Microbiology 51, no. 5 (1986) 985-989.

DOI: 10.1128/aem.51.5.985-989.1986

Google Scholar

[29] Couper, James R., W. Roy Penney, and James R. Fair. Chemical Process Equipment-Selection and Design (Revised 2nd Edition). Gulf Professional Publishing, (2009).

Google Scholar

[30] Petrides, Demetri., Bioprocess design and economics, in: Bioseparations Science and Engineering Second Edition, Oxford University Press, New York, 2000, pp.441-507.

Google Scholar

[31] Information on https://www.agaetech.com/.

Google Scholar

[32] Information on https://natsurfact.com/.

Google Scholar

[33] Sullivan, William G., Elin M. Wicks, and James T. Luxhoj. Engineering economy. Vol. 13. Upper Saddle River, NJ: Prentice Hall, (2003).

Google Scholar