Comparative Study of AFM and FESEM for Imaging the Single Cell of Escherichia Coli Bacteria

Abstract:

In this work, a comparative study using atomic force microscopy (AFM) and field emission–scanning electron microscopy (FESEM) has been carried out to assess the morphology of single cell Escherichia coli bacteria (E-coli). E-coli bacteria are a major concern for public health. Attention was focused on the certain strains of E-coli bacteria, because some strains can be toxic and cause food poisoning. The E-coli bacteria have attracted much research interest because this bacterium is easily to get, cheap and rapid reproductively. Imaging of E-coli recently, was improved by using high resolution microscopy. Current techniques for detection such as, AFM and FESEM has attracted great interest and emerging as a potentially powerful whole-organism fingerprinting tool for the rapid identification of bacteria. The obtained results of AFM and FESEM techniques have been compared to show the image quality of single cell E-coli.

• Periodical: Journal of Nano Research (Volume 34)
• Pages: 61-66
• DOI: https://doi.org/10.4028/www.scientific.net/JNanoR.34.61
• Online since: July 2015
• Authors: Kamarulazizi Ibrahim, Mohammad Hafiz Khalid, Mohamed Hassan Eisa*, Mohd Nazalan Najimudin, Mohammad A. Al Rajhi, Hajo Idriss
• Keywords: Atomic Force Microscopy, E-Coli Bacteria, Field Emission Scanning Electron Microscopy

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