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Exploiting the Sequence of Naturally Occurring Elastin: Construction, Production and Characterization of a Recombinant Thermoplastic Protein-Based Polymer

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Abstract:

Genetic engineering was used to produce an elastin-like polymer (ELP) with precise amino acid composition, sequence and length, resulting in the absolute control of MW and stereochemistry. A synthetic monomer DNA sequence encoding for (VPAVG)20, was used to build a library of concatemer genes with precise control on sequence and size. The higher molecular weight polymer with 220 repeats of VPAVG was biologically produced in Escherichia coli and purified by hot and cold centrifugation cycles, based on the reversible inverse temperature transition property of ELPs. The use of low cost carbon sources like lactose and glycerol for bacteria cells culture media was explored using Central Composite Design approach allowing optimization of fermentation conditions. Due to its self-assembling behaviour near 33 °C stable spherical microparticles with a size ~ 1µm were obtained, redissolving when a strong undercooling is achieved. The polymer produced showed hysteresis behaviour with thermal absorbing/releasing components depending on the salt concentration of the polymer solution.

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Journal of Nano Research Vol. 6 View Preview

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Periodical:

Journal of Nano Research (Volume 6)

Pages:

133-145

DOI:

https://doi.org/10.4028/www.scientific.net/JNanoR.6.133

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Online since:

June 2009

Authors:

Raul Machado, A.J. Ribeiro, J. Padrão, D. Silva, A. Nobre, J.A. Teixeira, F.J. Arias, António M. Cunha, José C. Rodríguez-Cabello, M. Casal

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Elastin-Like Polymer, Nanoparticle, Thermoplastic, Thermoresponsive Polymer, VPAVG

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© 2009 Trans Tech Publications Ltd. All Rights Reserved

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References

[1] A. Simnick, D. Lim, D. Chow, A. Chilkoti, Journal of Macromolecular Science, Part C: Polymer Reviews. 47 (2007) 121-154.

Google Scholar

[2] D.W. Urry, D.C. Gowda, T.M. Parker, C.H. Luan, M.C. Reid, C.M. Harris, A. Pattanaik, R.D. Harris, Biopolymers. 32 (1992) 1243-1250.

DOI: 10.1002/bip.360320913

Google Scholar

[3] R. Herrero-Vanrell, A.C. Rincón, M. Alonso, V. Reboto, I.T. Molina-Martinez, J.C. RodríguezCabello, Journal of Controlled Release. 102 (2005) 113-122.

DOI: 10.1016/j.jconrel.2004.10.001

Google Scholar

[4] J. Alper, Science. 297 (2002) 329-331.

Google Scholar

[5] K. Nagapudi, W.T. Brinkman, J. Leisen, B.S. Thomas, E.R. Wright, C. Haller, X. Wu, R.P. Apkarian, V.P. Conticello, E.L. Chaikof, Macromolecules. 38 (2005) 345-354.

DOI: 10.1021/ma0491199

Google Scholar

[6] A.C. Rincón, I.T. Molina-Martinez, B. de las Heras, M. Alonso, C. Baílez, J.C. RodíguezCabello, R. Herrero-Vanrell, Journal of Biomedical Materials Research Part A. 78A (2006) 343- 351.

DOI: 10.1002/jbm.a.30702

Google Scholar

[7] A. Girotti, J. Reguera, J.C. Rodríguez-Cabello, Journal of Materials Science: Materials in Medicine. 15 (2004) 479-484.

Google Scholar

[8] J.C. Rodríguez-Cabello, J. Reguera, A. Girotti, M. Alonso, A.M. Testera, Progress in Polymer Science. 30 (2005) 1119-1145.

Google Scholar

[9] F. Sanger, S. Nicklen, A.R. Coulson, Proceedings of the National Academy of Science. 74 (1977) 5463-5467.

Google Scholar

[10] H. Inoue, H. Nojima, H. Okayama, Gene. 96 (1990) 23-28.

Google Scholar

[11] J. Sambrook, D.W. Russel, T. Maniatis, Molecular Cloning: A Laboratory Manual, third ed., Cold Spring Harbour Laboratory Press, New York, (2001).

Google Scholar

[12] M.I. Viitanen, A. Vasala, P. Neubauer, T. Alatossava, Microbial Cell Factories. 2 (2003) 1-10.

DOI: 10.1186/1475-2859-2-2

Google Scholar

[13] D.C. Montgomery, Design and analysis of experiments, fifth ed., John Wiley & Sons Inc., New York, (2001).

Google Scholar

[14] L. Rodrigues, J. Teixeira, R. Oliveira, H.C. van der Mei, Process Biochemistry. 41 (2006) 1- 10.

Google Scholar

[15] J. Reguera, J.M. Lagarón, M. Alonso, V. Reboto, B. Calvo, J.C. Rodríguez-Cabello, Macromolecules. 36 (2003) 8470-8476.

DOI: 10.1021/ma034572q

Google Scholar

[16] J.C. Rodríguez-Cabello, J. Reguera, M. Alonso, T.M. Parker, D.T. McPherson, D.W. Urry, Chemical Physics Letters. 388 (2004) 127-131.

DOI: 10.1016/j.cplett.2004.03.013

Google Scholar

[17] J.C. Rodríguez-Cabello, Advances in Experimental Medicine and Biology. 553 (2004) 45-57.

Google Scholar

[18] P. Schmidt, J. Dybal, J.C. Rodríguez-Cabello, V. Reboto, Biomacromolecules. 6 (2005) 697- 706.

Google Scholar

[19] D.W. Urry, Journal of Physical Chemistry B. 101 (1997) 11007-11028.

Google Scholar

[20] J. Reguera, D.W. Urry, T.M. Parker, D.T. McPherson, J.C. Rodríguez-Cabello, Biomacromolecules. 8 (2007) 354-358.

Google Scholar

[21] Q. Zou, S.M. Habermann-Rottinghaus, K.P. Murphy, PROTEINS. 31 (1998).

Google Scholar