Protein Adsorption on Nanohydroxyapatites: Spectroscopic Investigations at Molecular Level

Luca Bertinetti; Raffaella Ceschino; Daniele Bollati; Elena Landi; Anna Tampieri; Gianmario Martra

doi:10.4028/www.scientific.net/KEM.396-398.77

Paper Titles

Comparative Effect of the Ionic Products from Bioactive Glass Dissolution on the Behavior of Cementoblasts, Osteoblasts, and Fibroblasts
p.55

In Vivo Behavior of Hydroxyapatite Evaluated by Attenuated Total Reflection Infrared Microscopy (ATR-FTIR)
p.61

Insulin Adsorption by Hydroxyapatite
p.65

Performance Evaluation of Hydroxyapatite Coatings Thermally Sprayed on Surgical Fixation Pins
p.69

Protein Adsorption on Nanohydroxyapatites: Spectroscopic Investigations at Molecular Level
p.77

Resorption Mechanism of Hydroxyapatite and β-Tricalcium Phosphate Coating Layer
p.81

The Importance of Osteoclast-Like Multinucleated Cells in Material-Induced Osteoinduction: A Comparative Study between Dogs and Rats
p.85

The Surface Property of Hydroxyapatite: Sensing with Quartz Crystal Microbalance
p.89

A Bioactive Glass/Dental Porcelain System by the Sol Gel Route: Fabrication and Characterization
p.95

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Protein Adsorption on Nanohydroxyapatites: Spectroscopic Investigations at Molecular Level

Abstract:

Proper thermal treatments allowed to modify the number of surface Ca2+ able to coordinate water molecules on the surface of hydroxyapatite (HA) nanoparticles surrounded by an amorphous layer. Despite the consequent significant difference in the first hydration level between untreated and treated HA, the amount of adsorbed BSA, used as a model protein, remained essentially unchanged and the native structure of adsorbed protein was retained (as indicated by mid-IR ATR). Near-IR spectroscopy evidenced that adsorbed proteins should be in direct contact with surface Ca2+ through a displacement of H2O molecules by charged acidic residues. In agreement with a previous study that evidenced the heterogeneity of surface Ca2+ ions in terms of Lewis acidity, it was then proposed that the adsorption of BSA on such nano-HA should be ruled by some feature of the local structure of surface Ca2+ sites, prevailing on the total number of cationic sites exposed and the related features of the first hydration layer.