Insulin Adsorption by Hydroxyapatite
In this study, the insulin adsorption ability by synthetic hydroxyapatite was investigated. Hydroxyapatite before thermal treatment and heated at 900°C and 1100°C were incubated in a solution containing 32 mg insulin/gHA. Adsorption and desorption experiments were carried out at 37°C under constant and slowly stirring. From FTIR results it was observed that insulin was tightly attached to HA surface after 96 hours adsorption experiment. Thermal pre-treatment on HA samples had no effect on insulin adsorption. However, protein desorption was more pronounced on samples not submitted to thermal treatment.
Marcelo Prado and Cecília Zavaglia
E. Mavropoulos et al., "Insulin Adsorption by Hydroxyapatite", Key Engineering Materials, Vols. 396-398, pp. 65-68, 2009