Adsorption of Proteins Derived from Fetal Bovine Serum onto Hydroxyapatite Nanocrystals with Quartz Crystal Microbalance Technique

Abstract:

The adsorption of multiple proteins derived from fetal bovine serum (FBS) in phosphate buffer saline (PBS) and alpha minimum essential (aMEM) was in situ analyzed with a quartz crystal microbalance with dissipation technique on gold, titanium and HAp sensors. The adsorption behaviors of FBS proteins were varied depending on the sensors. The DD/Df value of the HAp sensor were clearly different in PBS and aMEM, and others were not changed. The viscoelastic properties of the protein films adsorbed on the HAp sensor in PBS were flexible in comparison with those on the gold and titanium sensors. The D-f plots incidated that the proteins adsorbed on HAp in PBS would lead to competitive adsorption and conformational change and those in aMEM could form a monolayer. The adsorption behavior on the HAp in carbonate buffer saline was found to be similar to that in aMEM. These differential adsorption behaviors on the HAp surface were attributed to the pre-adsorptive ion, such PO43- or CO32- in the solvent.

• Periodical: Key Engineering Materials (Volumes 396-398)
• Pages: 47-50
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.396-398.47
• Online since: October 2008
• Authors: Motohiro Tagaya, Toshiyuki Ikoma, Taro Takemura, Mitsuhiro Okuda, Nobutaka Hanagata, Tomohiko Yoshioka, Dinko Chakarov, B. Kasemo, M. Tanaka
• Keywords: Biosensor, Fetal Bovine Serum (FBS), Hydroxyapatite (HAP), Protein Adsorption, QCM-D

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[50] 60 70 80 90 100 110 120 ∆ f n=3 / 3 (Hz) Time (min) Au Ti HAp.

[1] [2] [3] [4] [5] [6] [7] [8] [50] 60 70 80 90 100 110 120 ∆ D (106 ) Time (min) Au Ti HAp -80 -70 -60 -50 -40 -30 -20 -10.

[50] 60 70 80 90 100 110 120 ∆ f n=3 / 3 (Hz) Time (min) Au Ti HAp.

[1] [2] [3] [4] [5] [6] [7] [8] [50] 60 70 80 90 100 110 120 ∆ D (10 -6 ) Time (min) Au Ti HAp (a) (b) (c) (d) Figure 2 Frequency shifts (∆fn=3/3) and dissipation shifts (∆D) of the adsorption of the FBS proteins in PBS for (a) and (b) and in αMEM for (c) and (d) on the gold, titanium and HAp sensor.