Biochemical Investigation of Some Proteins from Human Primary Teeth to Evaluate Heavy Metal Pollution

Abstract:

This investigation is based on the premise that heavy metals are sequestered by the mineral phase of teeth [1,2], hydroxyapatite, during their formation and there is a relation between heavy metal pollution and progressive destruction of human primary tooth. Our study was carried out on temporary molars – sound and carious - extracted from children up to 6 year old, residing in an area of Romania known to be highly polluted with heavy metals (Pb and Cd). In carious dentin extracts we found increased levels of collagen type 1 and alkaline phosphatase activity that can be correlated with the remineralization processus during teeth decay. Gelatin zymography shown increased MMP-1 (type-I collagenase) and MMP-2 (gelatinase-A) activities only in dentin extract from carious teeth isolated from children residing in a highly polluted area, suggesting their role in pathological extracellular matrix (ECM) remodeling and degradation processes in the progression of carious decay.

• Periodical: Key Engineering Materials (Volume 415)
• Pages: 53-56
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.415.53
• Online since: September 2009
• Authors: R. Ciubar Ion, C.M. Ciofrangeanu, B. Galateanu, Stef Manea, A. Cimpean, D. Iordǎchescu
• Keywords: Alkaline Phosphatase, Collagen Type 1, Dental Caries, Heavy Metal, MMP-1, MMP-2

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