Effect of Lithium Chloride and Sodium Propionate on Growth of Selected Probiotics

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Effect of Lithium Chloride (LiCl) and Sodium Propionate (CH3CH2COONa) on growth of Lactobacillus acidophilus, Bifidobacterium bifidum, Lactobacillus bulgaricus, Lactobacillus casei and Lactobacillus rhamnosus was studied by measuring optical density at 600nm (OD600) and pH using MRS media as the control. The addition of each substance (%, w/v) was 0.025%, 0.05%, 0.1%, 0.2% and 0.3%. Results were as follows: addition of LiCl has the significant inhibition on growth of Lactobacillus acidophilus, Bifidobacterium bifidum and Lactobacillus bulgaricus at incubation 12h. The optimum selective concentration of LiCl in MRS media was 0.1% both for Lactobacillus acidophilus and Lactobacillus bulgaricus. Lactobacillus rhamnosus can tolerate the substances mentioned above. Sodium Propionate has no effect for any of five strains.

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Key Engineering Materials (Volumes 480-481)

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66-69

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June 2011

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© 2011 Trans Tech Publications Ltd. All Rights Reserved

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[1] Fuller R: Probiotics in man and animals. Journal of Applied Bacteriology. Vol. 66(1989), p.365.

Google Scholar

[2] Gismondo M R, Drago L and Lombardi: A Review of probiotics available to modify gastrointestinal flora. International Journal of Antimicrobial Agent. Vol. 12(1999), p.287.

DOI: 10.1016/s0924-8579(99)00050-3

Google Scholar

[3] Holzaspfel W H and Schillinger U: Introduction to pre-and probiotics, Food Research International . Vol. 35(2001), p.109.

Google Scholar

[4] Shah N P: Functional foods from probiotics and prebiotics. Food Technology. Vol. 55 (2001), p.46.

Google Scholar

[5] N. Tharmaraj and N. P. Shah: Selective Enumeration of Lactobacillus delbrueckii ssp. bulgaricus, Streptococcus thermophilus, Lactobacillus acidophilus, Bifdobacteria, Lactobacillus casei, Lactobacillus rhamnosus and Propionibacteria.J. DairySci. Vol. 86(2003).

DOI: 10.3168/jds.s0022-0302(03)73821-1

Google Scholar

[6] de Man, J. C., Rogosa, M., a Sharpe, M. E.: Applied Bacteriology. Vol. 23(1960), p.130.

Google Scholar

[7] Laroia, S., a Martin, J. H.: Methods for enumerating bifidobacteria. Cultured Dairy Products Journal. Vol. 5(1991b), p.32.

Google Scholar

[8] Terzaghi, B. E., aSandine, W. E.: Improved medium for lactic streptococci and their bacteriophages. Applied Microbiology, Vol. 29 (1975), p.807.

DOI: 10.1128/am.29.6.807-813.1975

Google Scholar

[9] Teraguchi, S., Uehara, M., Ogasa, K., a Mitsuoka, T.: Enumeration of Bifidobacteria in dairy products, Japanese Journal of Bacteriology. Vol. 33 (1978), p.753.

Google Scholar

[10] Onggo,I., and G. H. Fleet: Media for the isolation and enumeration of lactic acid bacteria from yoghurts. Aust.J. DairyTechnol. Vol. 48 (1993), p.89.

Google Scholar

[11] Samona,A., and R.K. Robinson: Effect of yoghurt cultures on the survival of bifdobacteria in fermented milks.J. Soc. DairyTechnol. Vol. 47 (1994), p.58.

Google Scholar