Fabrication of Organic-Inorganic Hybrid Nanopore and its Application in Biosensing

Abstract:

Nanopore and nanopore based bio-sensing technology have become into more and more interesting research area in the past ten years. In this work, micro-pore in Si-S₃N₄ chips was fabricated and characterized by Focused Ion Beam (dual Beam), and then the S₃N₄ pore was covered by Polycarbonate (PC) membrane containing 50nm nanopores and sealed by using polydimethylsiloxane (PDMS) to get hybrid micro-nanopores. The obtained chip with hybrid nanopores together with two liquid cells was integrated into an ionic current detection device for biosensing. Based on this device, λ-DNA in the electrolytic solution can be detected when it is electrophoretically driven through the hybrid nanopores, and different gestures of λ-DNA in translocation also can be discriminated.

• Periodical: Key Engineering Materials (Volumes 562-565)
• Pages: 658-663
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.562-565.658
• Online since: July 2013
• Authors: Lei Liu, Bing Wang, Yue Yang, Zhong Hua Ni, Yun Fei Chen
• Keywords: DNA, Nanopore, Sensing

References

[1] J.J. Kasianowicz, E. Brandin, D. Branton and D.W. Deamer, Characterization of individual polynucleotide molecules using a membrane channel, Proc. Natl. Acad. Sci. USA 93(1996), 13770–13773.

DOI: 10.1073/pnas.93.24.13770

[2] G.V. Soni, C. Dekker, Detection of Nucleosomal Substructures using Solid-State Nanopores, Nano Lett. 12(2012), 3180-3186.

DOI: 10.1021/nl301163m

[3] J.K. Rosenstein, M. Wanunu, C.A. Merchant, M. Drndic, K.L. Shepard, Integrated nanopore sensing platform with sub-microsecond temporal resolution, Nature Methods, 9(2012).487-U112

DOI: 10.1038/nmeth.1932

[4] S. Das, P. Dubsky, A. van den Berg, J.C.T. Eijkel, Concentration Polarization in Translocation of DNA through Nanopores and Nanochannels, Phy. Rev. Lett. 108(2012), 138101

DOI: 10.1103/physrevlett.108.138101

[5] H. Bayley, P.S. Cremer. Stochastic sensors inspired by biology, Nature, 413(2001), p.226–230.

DOI: 10.1038/35093038

[6] H. Bayley, L. Jayasinghe, Functional engineered channels and pores - (Review), Molecular Membrane Biology 21(2004), 209–220.

DOI: 10.1080/09687680410001716853

[7] Q. Jin, A. M. Fleming, C. J. Burrows, Unzipping Kinetics of Duplex DNA Containing Oxidized Lesions in an alpha-Hemolysin Nanopore, J. Am. Chem. Soc. 134(2012): 11006-11011

DOI: 10.1021/ja304169n

[8] S. Wen, T. Zeng, L.Liu, Highly Sensitive and Selective DNA-Based Detection of Mercury(II) with alpha-Hemolysin Nanopore, J. Am. Chem. Soc., 133(2011): 18312-18317

DOI: 10.1021/ja206983z

[9] R.S.S. de Zoysa, D.M.M. Krishantha, Q.Zhao, Translocation of single-stranded DNA through the alpha-hemolysin protein nanopore in acidic solutions, Electrophoresis, 32 (2011), 3034-3041

DOI: 10.1002/elps.201100216

[10] J. Li, D. Stein, C. McMullan, D. Branton, M. J. Aziz and J. A. Golovchenko, Ion-beam sculpting at nanometre length scales, Nature, 2001, 412, 166–169.

DOI: 10.1038/35084037

[11] J. Li, M. Gershow, D. Stein, E. Brandin and J. A. Golovchenko, DNA molecules and configurations in a solid-state nanopore microscope, Nat. Mater., 2003, 2, 611–615.

DOI: 10.1038/nmat965

[12] B. Lu, D. P. Hoogerheide, Q. Zhao, Effective driving force applied on DNA inside a solid-state nanopore, Phy. Rev. E, 011921(2012)

DOI: 10.1103/physreve.86.011921

[13] M. Wanunu, S.Bhattacharya, Y. Xie, Y. Tor, A. Aksimentiev, M. Drndic, Nanopore Analysis of Individual RNA/Antibiotic Complexes, ACS NANO, 5(2011): 9345-9353

DOI: 10.1021/nn203764j

[14] R.S. Wei, V. Gatterdam, R.Wieneke, Stochastic sensing of proteins with receptor-modified solid-state nanopores, Nature Nanotechnol. 7(2012), 257-263

DOI: 10.1038/nnano.2012.24

[15] P. S. Spinney, D. G. Howitt, R. L.Smith, Nanopore formation by low-energy focused electron beam machining, Nanotechnology, 21(2010), 375301

DOI: 10.1088/0957-4484/21/37/375301

[16] C. M. Edmonds, Y. C. Hudiono, A. G. Ahmadi, Polymer translocation in solid-state nanopores: Dependence of scaling behavior on pore dimensions and applied voltage, J. Chem. Phy. 136(2012), 065105

DOI: 10.1063/1.3682777

[17] Q. Zhao, Y. Wang, J. J. Dong, Nanopore-Based DNA Analysis via Graphene Electrodes, J. Nanomater., 318950(2012)

[18] B. M. Venkatesan, D. Estrada, S. Banerjee, Stacked Graphene-Al2O3 Nanopore Sensors for Sensitive Detection of DNA and DNA-Protein Complexes, ACS NANO, 6(2012), 441-450

DOI: 10.1021/nn203769e

[19] K. K. Saha, M. Drndic, B. K. Nikolic, DNA Base-Specific Modulation of Microampere Transverse Edge Currents through a Metallic Graphene Nanoribbon with a Nanopore, Nano Lett. 12(2012) 50-55

DOI: 10.1021/nl202870y

[20] A.J. Storm, J.H. Chen, H.W. Zandbergen, Translocation of double-strand DNA through a silicon oxide nanopore, Phy. Rev. E, 71(2005), 051903

DOI: 10.1103/physreve.71.051903

[21] H. Chang, F. Kosari, G. Andreadakis, DNA-mediated fluctuations in ionic current through silicon oxide nanopore channels, Nano Lett., 4(2004), 1551-1556

DOI: 10.1021/nl049267c

[22] D. Dobrev, J. Vetter, R. Neumann and N. Angert, Conical etching and electrochemical metal replication of heavy-ion tracks in polymer foils, J. Vac. Sci. Technol., B, 19(2001), 1385–1387.

DOI: 10.1116/1.1381066

[23] Z. Siwy, P. Apel, D. Baur, D. D. Dobrev, Y. E. Korchev, R. Neumann, R. Spohr, C. Trautmann and K. O. Voss, Surf. Sci., 532–535(2003), Preparation of synthetic nanopores with transport properties analogous to biological channels, 1061–1066

DOI: 10.1016/s0039-6028(03)00448-5

[24] C. C. Harrell, Y. Choi, L. P. Horne, L. A. Baker, Z. S. Siwy and C. R. Martin, Resistive-pulse DNA detection with a conical nanopore sensor, Langmuir, 22(2006), 10837–10843

DOI: 10.1021/la061234k

[25] A. Mara, Z. Siwy, C. Trautmann, J. Wan and F. Kamme, An asymmetric polymer nanopore for single molecule detection, Nano Lett., 4(2004), 497–501.

DOI: 10.1021/nl035141o