A Novel Conjugated Polyfluorene: Synthesis, Characterization and Application in Flow Injection Electrochemiluminescence for Detection of Bovine Serum Albumin

Abstract:

Driven by the urgent demand for novel electrochemiluminescence reagents, a water-soluble polyfluorene derivative, poly [(9,9-bis (3-((N,N-dimethylamino)N-ethy-lammonium) propyl)-2,7-fluorene)-alt-2,7-(9,9-p-divinylbenzene)] dibromide (P-2), was designed and success-fully synthesized. Ultraviolet-visible spectroscopy and fluorescence spectroscopy were used to characterize the formation of P-2, and the results showed that P-2 had strong fluorescence intensity. More importantly, electrochemiluminescence (ECL) of the prepared P-2 was also observed when the applied potential was cycled between 1.0 to 2.8 V at 100 mV·s^-1 in phosphate buffered saline buffer solution (pH 8.20). Based on the stable ECL performance, an ECL system for bovine serum albumin (BSA) was constructed. Under the optimal conditions, the enhanced ECL intensity was linearly correlated to the concentration of BSA over the range of 5.0×10^-8-1×10^-4 g·mL^-1 (R=0.9996) with a detection limit of 1.26×10^-8 g·mL^-1 (n=11). This work not only introduced a substitute for traditional ECL regents, but also proposed a simple ECL system for other analytes.

• Periodical: Key Engineering Materials (Volumes 575-576)
• Pages: 107-110
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.575-576.107
• Online since: September 2013
• Authors: Shu Ai Li, Shao Wei Wang, Cheng Chao Chu, Xiu Wang, Mei Yan, Jing Hua Yu, Sheng Guang Ge
• Keywords: Bovine Serum Albumin, Flow Injection Electrochemiluminescence, Polyfluorene Derivative

References

[1] H. Cui, G.Z. Zou, X.Q. Lin, Electrochemiluminescence of luminol in alkaline solution at a paraffin-impregnated graphite electrode, Anal. Chem. 75 (2003) 324-331.

DOI: 10.1021/ac0201631

[2] N.E. Tokel, A.J. Bard, Electrochemistry and emission from sytems containing Tris(2, 2'-bipyridine)ruthenium(II) dichloride, J. Am. Chem. Soc. 94 (1972) 2862-2863.

DOI: 10.1021/ja00763a056

[3] S.W. Wang, J.H. Yu, F.W. Wan, S.G. Ge, M. Yan, M. Zhang, Determination of sibutramine with a new sensor based on luminol electrochemiluminescence, J. Lumin. 131 (2011) 1515-1519.

DOI: 10.1016/j.jlumin.2011.03.038

[4] U. Janakiraman, D. Dini, A. Preusser, A.B. Holmes, R.E. Martin, K. Doblhofer, Electrochemiluminescence of conjugated polymer, Synth. Met. 121 (2001) 1685-1686.

DOI: 10.1016/s0379-6779(00)01122-x

[5] M. Bernius, M. Inbasekaran, E. Woo, W. Wu, L. Wujkowski, Light-emitting diodes based on fluorene polymers, Thin Solid Films 363 (2000) 55-57.

DOI: 10.1016/s0040-6090(99)00982-7

[6] R.I. Stankovic, R.W. Lenz, F.E. Karasz, Electrical conductivity of poly(butadiene-co-2-vinyl pyridine) diblock copolymers doped with TCNQ, Eur. Polym. J. 26 (1990) 675-681.

DOI: 10.1016/0014-3057(90)90227-u

[7] Gao W.Q., Yan M., Ge S.G., Liu X.X., Yu J.H., Fluorescent sensor based on a novel conjugated polyfluorene derivative, Spectrochim. Acta, Part A 95 (2012) 218-223.

DOI: 10.1016/j.saa.2012.04.027