Paper Titles
Nano-Hybrid-Composite Scaffolds from Substituted Apatite/Gelatin
p.233
Preparation and Characterization of Collagen/Hydroxyapatite Microsphere Composite Scaffold for Bone Regeneration
p.239
Silk Fibroin/Nano-CaP Bilayered Scaffolds for Osteochondral Tissue Engineering
p.245
Development of a Novel Hybrid Porous Scaffold for Bone Tissue Engineering: Forsterite Nanopowder Reinforced Chitosan
p.249
Gellan Gum-Based Hydrogel Bilayered Scaffolds for Osteochondral Tissue Engineering
p.255
Recent Progress in the Field of Multicomponent Biocompatible Nanostructured Films
p.263
Bioactive Ti Metal with Ca-Enriched Surface Layer Able to Release Sr Ions
p.269
TiSiN Coatings for Improved Bond Strength of CoCr Alloy to Dental Ceramic
p.275
CO2 Laser Assisted Processing of Bioactive Titanium-Based Surfaces
p.282

Gellan Gum-Based Hydrogel Bilayered Scaffolds for Osteochondral Tissue Engineering

Article Preview

Abstract:

It has been shown that hydrogel bilayered scaffolds combining cartilage- and bone-like layers are most advantageous for treating osteochondral defects. In this study, it is proposed the use of low acyl gellan gum (LAGG) for developing bilayered hydrogel scaffolds for osteochondral tissue engineering. The cartilage-like layer of the GG-based bilayered hydrogel scaffolds is composed of LAGG (2 wt%). By adding a 2 wt% LAGG aqueous solution to different amounts of HAp (5-20 wt%) it was possible to produce the bone-like layer. In vitro bioactivity tests were performed by means of soaking the LAGG/LAGG-HAp hydrogel scaffolds in a simulated body fluid solution up to 14 days. Scanning electron microscopy, Fourier transform infra-red spectroscopy and X-ray diffraction analyses demonstrated that apatite formation is limited to the bone-like layer of the LAGG/LAGG-HAp bilayered hydrogel scaffolds.

You might also be interested in these eBooks
Bioceramics 25 View Preview

Info:

Periodical:

Key Engineering Materials (Volume 587)

Pages:

255-260

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.587.255

Citation:

Cite this paper

Online since:

November 2013

Authors:

Diana R. Pereira, Raphael F. Canadas, Joana Silva-Correia, Alexandra P. Marques, Rui L. Reis, Joaquim Miguel Oliveira

Keywords:

Bilayered Scaffold, Gellan Gum, Hydrogel, Osteochondral, Tissue Engineering

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

© 2014 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

References

[1] P.B. Malafaya, R.L. Reis. Bilayered chitosan-based scaffolds for osteochondral tissue engineering: Influence of hydroxyapatite on in vitro cytotoxicity and dynamic bioactivity studies in a specific double-chamber bioreactor, Acta. Biomater. 5(2009).

DOI: 10.1016/j.actbio.2008.09.017

Google Scholar

[2] J.M. Oliveira, M.T. Rodrigues, S.S. Silva, P.B. Malafaya, M.E. Gomes, C.A. Viegas, et al. Novel hydroxyapatite/chitosan bilayered scaffold for osteochondral tissue-engineering applications: Scaffold design and its performance when seeded with goat bone marrow stromal cells, Biomaterials. 27(2006).

DOI: 10.1016/j.biomaterials.2006.07.034

Google Scholar

[3] J.T. Oliveira, L.S. Gardel, T. Rada, L. Martins, M.E. Gomes, R.L. Reis. Injectable gellan gum hydrogels with autologous cells for the treatment of rabbit articular cartilage defects, J. Orthop. Res. 28(2010) 1193-9.

DOI: 10.1002/jor.21114

Google Scholar

[4] J.T. Oliveira, T.C. Santos, L. Martins, R. Picciochi, A.P. Marques, A.G. Castro, et al. Gellan gum injectable hydrogels for cartilage tissue engineering applications: In vitro studies and preliminary in vivo evaluation, Tissue Eng. Part A. 16(2010).

DOI: 10.1089/ten.tea.2009.0117

Google Scholar

[5] J.T. Oliveira, T.C. Santos, L. Martins, M.A. Silva, A.P. Marques, A.G. Castro, et al. Performance of new gellan gum hydrogels combined with human articular chondrocytes for cartilage regeneration when subcutaneously implanted in nude mice, J. Tissue. Eng. Regen. Med. 3(2009).

DOI: 10.1002/term.184

Google Scholar

[6] J. Silva-Correia, J.M. Oliveira, S.G. Caridade, J.T. Oliveira, R.A. Sousa, J.F. Mano, et al. Gellan gum-based hydrogels for intervertebral disc tissue-engineering applications, J. Tissue. Eng. Regen. Med. 5(2011) e97-107.

DOI: 10.1002/term.363

Google Scholar

[7] J.M. Oliveira, S.S. Silva, P.B. Malafaya, M.T. Rodrigues, N. Kotobuki, M. Hirose, et al. Macroporous hydroxyapatite scaffolds for bone tissue engineering applications: Physicochemical characterization and assessment of rat bone marrow stromal cell viability, J. Biomed. Mater. Res. Part A. 91(2009).

DOI: 10.1002/jbm.a.32213

Google Scholar

[8] T. Kokubo, H. Kushitani, S. Sakka, T. Kitsugi, T. Yamamuro. Solutions able to reproduce in vivo surface-structure changes in bioactive glass-ceramic A-W, J. Biomed. Mater. Res. 24(1990) 721-34.

DOI: 10.1002/jbm.820240607

Google Scholar