Experimental Investigation of Cell Cloning Efficiency after Optical Trapping

Kozo Taguchi; Takuya Hayashi

doi:10.4028/www.scientific.net/KEM.625.695

Paper Titles

Design Life Analysis of KTX Running Gear Systems on Various Operating Conditions
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Dielectrophoresis Tweezers for Single Cell Manipulation Using Metal Coated Chemically Etched Fiber
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Dynamic Performance Measurement of Proximity Sensors for a Mobile Robot
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Evaluation of Running Safety for Railway vehicles Based on Vibration Acceleration of Bogie
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Experimental Investigation of Cell Cloning Efficiency after Optical Trapping
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Numerical Simulation Research of an Ultra-Micro Dispensing
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Optical Simulation of Omnidirectional Inspection System for Screw Threads
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Study of Mechanism for Wear Adjustment with Electro Wedge Brake
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Swirl-Based Control Method of Cell Orientation-Impact on Control Performance with Eccentricity
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HomeKey Engineering MaterialsKey Engineering Materials Vol. 625Experimental Investigation of Cell Cloning...

Experimental Investigation of Cell Cloning Efficiency after Optical Trapping

Abstract:

In this paper, chemically etched axicon fiber was investigated for laser trapping of micro-object dispersed in liquid. We fabricated axicon micro lenses on a single-mode bare optical fiber by selective chemical etching technique. The laser beam from fiber axicon microlens was strongly focused and optical forces were sufficient to move a microorganisms and biological cells without physical contact. In our experiments, several different lasers with various wavelengths were used as light sources. From these experimental results, it was found that laser wavelength was very important parameter for cell trapping and laser wavelength should be selected to avoid absorption by cells in order to prevent thermal degradation and damage to the cells.

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Periodical:

Key Engineering Materials (Volume 625)

Pages:

695-699

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.625.695

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Online since:

August 2014

Authors:

Kozo Taguchi*, Takuya Hayashi

Keywords:

Biological Cells, Chemical Etching, Fiber Axicon, Laser Trapping, Optical Fiber

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References

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