Dielectrophoresis Tweezers for Single Cell Manipulation Using Metal Coated Chemically Etched Fiber

Abstract:

We proposed a simple and low cost dielectrophoretic device to trap and isolate single target cells. The device consisted of a metal coated chemically etched fiber and an AC signal generator. It did not require microfabrication technologies or sophisticated electronics. Using this system, we could easily trap and isolate yeast cells at will. Furthermore, our dielectrophoretic manipulator also could discriminate between live and dead cells by tuning of the applied signal frequency. From these experimental investigations, it was found that our proposed dielectrophoresis tweezers using metal coated chemically etched fiber was a promising tool for the single cell manipulation and isolation.

• Periodical: Key Engineering Materials (Volume 625)
• Pages: 678-682
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.625.678
• Online since: August 2014
• Authors: Kozo Taguchi*, Keishu Aritoshi, Kyohei Nishimoto, Shun Fukutomi
• Keywords: Biological Cell, Dielectrophoresis Tweezers, Isolation, Manipulation

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