The Measurement of Erythrocyte Size by Using Diffraction Technique Base on Laser

Suebtarkul Suchat; Chantira Boonrod

doi:10.4028/www.scientific.net/KEM.675-676.718

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The Measurement of Erythrocyte Size by Using Diffraction Technique Base on Laser

Abstract:

The measurement of erythrocyte size was studied by using diffraction technique base on laser. The light from a diode laser was observed with 650 nm and 532 nm of light wavelength through erythrocyte on the slide. The result found that the ring was appeared on the screen alternate with brightness and dark. The first brightness radius was measured on the screen (Y) and the distance of the position of the slide erythrocytes to screen (X) was observed then calculated the diameter of an erythrocyte (d) in microns (μm). The above number were calculated the volume of erythrocyte and compared with automatically complete blood count (CBC) machine by the mean corpuscular volume (MCV) with femtolitre (fl) values in the range 60-100 femtolitre. In conclusion, this study showed that the sizes of erythrocyte by laser diffraction technique are similar with the automated blood count. Therefore, it can be applied to measure erythrocyte size.

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Periodical:

Key Engineering Materials (Volumes 675-676)

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718-721

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.675-676.718

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Online since:

January 2016

Authors:

Suebtarkul Suchat*, Chantira Boonrod

Keywords:

Diffraction, Erythrocyte, Optical Measurement

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