Immobilization of Laccase in Glycidoxypropyl-Functionalized Magnetic Mesoporous SiO₂/Fe₃O₄ Hollow Microspheres

Abstract:

Glycidoxypropyl-functionalized magnetic mesoporous SiO₂/Fe₃O₄ hollow microspheres (FMMS) with various amounts of terminally bonded groups in the pore channels were synthesized by the co-condensation of tetraethoxysilane (TEOS) and 3-glycidoxypropyltrimethoxylsilane (GPTMS) in the presence of cetyltrimethylammonium bromide (CTAB) and 1,3,5-triisoporpylbenzene (TMB), and utilized as supports for laccase immobilization. It is revealed that glycidoxypropyl groups have been successfully covalently attached to the pore wall of magnetic mesoporous SiO₂/Fe₃O₄ hollow microspheres (MMS). The magnetic mesoporous SiO₂/Fe₃O₄ microspheres (sample10%FMMS) have a saturation magnetization of 13.6 emu·g^-1 and a low coercivity (50 Oe). The functional materials preserve a mesoscopic ordering at a concentration of GPTMS as high as 15%. The BET surface area and pore volume of the functionalized materials decrease with increasing amount of GPTMS, but a desirable pore structure remains when the GPTMS amount increases to 10%. The 10%FMMS material exhibits higher laccase immobilization and stability than pure MMS because of the covalent interaction between the amino groups of laccase and the epoxy groups of the functional MMS.

• Periodical: Key Engineering Materials (Volume 697)
• Pages: 749-755
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.697.749
• Online since: July 2016
• Authors: Peng Yan Wang, Qun Yan Li*, Yun Lu Zhou, Qi Wei, Zuo-Ren Nie
• Keywords: Glycidoxypropyl-Functionalized, Laccase, Mesoporous SiO₂/Fe₃O₄

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