Compound Mutation Breeding of Streptococcus equi JC-63 for Producing High Molecular Weight Hyaluronic Acid and Optimization of Culture Conditions

Rong Hu Zhang; Jian Cheng Feng

doi:10.4028/www.scientific.net/KEM.703.337

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Compound Mutation Breeding of Streptococcus equi JC-63 for Producing High Molecular Weight Hyaluronic Acid and Optimization of Culture Conditions

Abstract:

A strain of mutant Streptococcus equi JC-63, which can produce high molecular weight hyaluronic acid, was obtained from compound mutation breeding of Streptococcus equi JC-0 using 5-Bromouracil, UV rays and nitrosoguanidine as mutagenic agents. After inoculation for 25 generations, Streptococcus equi JC-63 showed inheritance stability, but not hemolysis. The culture conditions for producing HMW-HA were optimized in a 1-L shake flask through multi-factor orthogonal tests. Results showed the optimum ratio of fermentation medium comprised 50 g/L sucrose, 80 g/L corn steep liquor, 10 g/L glucose, 2 g/L MgSO₄, 2 g/L NaHCO₃, 2 g/L Na₂HPO₄, and 0.1 g/L UTP. Under these fermentation conditions, the molecular weight of the produced HA was 2.76 × 10⁶ Da, and HA yield reached 4.12 g/L.

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Advanced Materials and Engineering Materials V

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Key Engineering Materials (Volume 703)

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337-342

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https://doi.org/10.4028/www.scientific.net/KEM.703.337

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August 2016

Authors:

Rong Hu Zhang, Jian Cheng Feng*

Keywords:

Compound Mutation, Culture Conditions, High Molecular Weight, Hyaluronic Acid, Streptococcus equi

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